Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

被引：46

作者：

Li, Zheng ^{[1
]}

Liu, Dan ^{[1
]}

Xia, Yu ^{[1
]}

Li, Ziliang ^{[1
]}

Jing, Doudou ^{[1
]}

Du, Jingjing ^{[1
]}

Niu, Na ^{[1
]}

Ma, Shoucai ^{[1
]}

Wang, Junwei ^{[1
]}

Song, Yulong ^{[1
]}

Yang, Zhiquan ^{[1
]}

Zhang, Gaisheng ^{[1
]}

机构：

[1] Northwest A&F Univ, Minist Educ,Natl Yangling Agr Biotechnol & Breedi, State Wheat Improvement Ctr,Wheat Breeding Engn R, Coll Agron,Yangling Branch,Key Lab Crop Heterosis, Yangling 712100, Shaanxi, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 05期

关键词：

WOX gene family; flower development; interaction analysis; TaWOX9; wheat; TRANSCRIPTION FACTOR; ROOT DEVELOPMENT; FLORAL ORGAN; CELL FATE; ARABIDOPSIS; PROTEIN; TILLER; RICE; EXPRESSION; SHOOT;

D O I：

10.3390/ijms21051581

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The WUSCHEL-related homeobox (WOX) is a family of plant-specific transcription factors, with important functions, such as regulating the dynamic balance of division and differentiation of plant stem cells and plant organ development. We identified 14 distinct TaWOX genes in the wheat (Triticum aestivum L.) genome, based on a genome-wide scan approach. All of the genes under evaluation had positional homoeologs on subgenomes A, B and D except TaWUS and TaWOX14. Both TaWOX14a and TaWOX14d had a paralogous copy on the same genome due to tandem duplication events. A phylogenetic analysis revealed that TaWOX genes could be divided into three groups. We performed functional characterization of TaWOX genes based on the evolutionary relationships among the WOX gene families of wheat, rice (Oryza sativa L.), and Arabidopsis. An overexpression analysis of TaWUS in Arabidopsis revealed that it affected the development of outer floral whorl organs. The overexpression analysis of TaWOX9 in Arabidopsis revealed that it promoted the root development. In addition, we identified some interaction between the TaWUS and TaWOX9 proteins by screening wheat cDNA expression libraries, which informed directions for further research to determine the functions of TaWUS and TaWOX9. This study represents the first comprehensive data on members of the WOX gene family in wheat.

引用

页数：17

共 43 条

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