IN VITRO GERMINATION AND ACCLIMATION OF SUCUPIRA-PRETA SEEDLINGS (Bowdichia virgilioides Kunth.)

被引:0
|
作者
de Moura, Luciana Coelho [1 ]
Titon, Miranda [1 ]
Cunha Fernandes, Jose Sebastiao [1 ]
Santana, Reynaldo Campos [1 ]
机构
[1] Univ Fed Vales Jequitinhonha & Mucuri UFVJM, Diamantina, MG, Brazil
来源
BIOSCIENCE JOURNAL | 2014年 / 30卷 / 05期
关键词
Bowdichia virgilioides Kunth; Tissue culture; Culturemedium; Plantpropagation; CULTURE; SEEDS;
D O I
暂无
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Thiss tudy objectived to evaluate the in vitro germination and acclimatization of plants sucupirapreta (Bowdichia virgilioides). Scarified and not scarified seeds were inoculated in test tubes containing culture medium MSorWPM, 100 or 50% of salts and vitamins, supplemented with two types of additives, activated charcoal and PVP, and kept in culture room. We used acompletely randomized in a 2x4 factorial design (2additives and 4culture medium formulations) with 4 replications and6 seeds per replicate. At 30 days gave the germination percentage and germination speed index(IVG). Plants were transferred to tubes containing substrate and acclimatized in a greenhouse, where they remained for 60days and transferred to a dusk house, where they remained for another 60days. At 30, 60, 90 and 120 days, we assessed the percentage of survival and seedling height acclimated. The in vitro germination of scarified seeds had the highest percentage using culture media MS and WPM halved the concentration of salts and vitamins. Germination occurred regardless of additive used both to scarified and note scarified seeds. The acclimation of plants germinated in vitro occurred regardless of additives history or culture media used.
引用
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页码:678 / 687
页数:10
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