Membrane protein structural biology in the era of single particle cryo-EM

被引：102

作者：

Cheng, Yifan ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2018年 / 52卷

关键词：

ELECTRON CRYOMICROSCOPY STRUCTURE; BEAM-INDUCED MOTION; ION-CHANNEL; CRYSTAL-STRUCTURE; ATOMIC-STRUCTURE; CRYOELECTRON MICROSCOPY; TRPV1; STRUCTURES; RECEPTOR; ARCHITECTURE; COMPLEX;

D O I：

10.1016/j.sbi.2018.08.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the past few years, significant technological breakthroughs in single particle cryo-electron microscopy enabled a `resolution revolution' of this technique. It also changed structural biology in an 'unprecedented way. For many biological macromolecules, obtaining well-ordered crystals of suitable size is no longer a prerequisite for determining their atomic structures. One of the most impacted areas is the structural biology of integral membrane proteins. New structures are now determined at a rapid pace. Despite these advances, further technological developments are still required to overcome new technical challenges that face membrane protein structural biology. In this review, I attempt to discuss some of these challenges.

引用

页码：58 / 63

页数：6

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