LncRNA TMPO-AS1 serves as a sponge for miR-4731-5p modulating breast cancer progression through FOXM1

被引：1
作者：
Wang, Ying [1 ,2 ]
Ma, Jun [3 ]
Li, Ruiqing [2 ]
Gao, Xiaokang [2 ]
Wang, Hongli [2 ]
Jiang, Guoqin [1 ]
机构：
[1] Soochow Univ, Affiliated Hosp 2, Dept Surg, 1055 Sanxiang Rd, Suzhou 215004, Jiangsu, Peoples R China
[2] Yangzhou Univ, Dept Gen Surg, Affiliated Hosp, Yangzhou 225012, Jiangsu, Peoples R China
[3] Anqing Municipal Hosp, Dept Surg Oncol, Anqing 246003, Anhui, Peoples R China
来源：
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2021年 / 13卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Long noncoding RNAs; TMPO-AS1; breast cancer; miR-4731-5p; FOXM1; LONG NONCODING RNA; REPRESSION; ACTIVATION; PROMOTES; ROLES;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective: To investigate the function of IncRNA-TMPO-AS1 in breast cancer (BC) and to further explore its molecular mechanism. Methods: TMPO-AS1, miR-4731-5p and FOXM1 were quantitatively determined using qRT-PCR. CCK-8 assays, plate cloning experiments, wound healing and Transwell assays, and flow cytometry were used to assess the biological behaviors of BC cells. Dual-luciferase reporter assays were used to assess the interactions between TMPO-AS1 and its downstream targets. The apoptosis and cell cycle-related proteins were quantitatively determined using Western blot. Results: In the BC tissues and cells, TMPO-AS1 was significantly increased (P<0.05). Functional studies suggest that the knockdown of TMPO-AS1 tremendously restrains tumor cell growth and migration (P<0.05). Mechanically, TMPO-AS1 negatively regulates miR-4731-5p and influences the progression of BC through the miR-4731-5p/FOXM1 axis. Conclusion: LncRNA TMPO-AS1spongess miR-4731-5p to modulate BC progression through FOXM1.
引用
收藏
页码：11094 / 11106
页数：13
相关论文
共 27 条
[1]   Targeting noncoding RNAs in disease [J].
Adams, Brian D. ;
Parsons, Christine ;
Walker, Lisa ;
Zhang, Wen Cai ;
Slack, Frank J. .
JOURNAL OF CLINICAL INVESTIGATION, 2017, 127 (03) :761-771
[2]   Advanced Breast Cancer and Breast Cancer Mortality in Randomized Controlled Trials on Mammography Screening [J].
Autier, Philippe ;
Hery, Clarisse ;
Haukka, Jari ;
Boniol, Mathieu ;
Byrnes, Graham .
JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (35) :5919-5923
[3]   A Long Noncoding RNA Mediates Both Activation and Repression of Immune Response Genes [J].
Carpenter, Susan ;
Aiello, Daniel ;
Atianand, Maninjay K. ;
Ricci, Emiliano P. ;
Gandhi, Pallavi ;
Hall, Lisa L. ;
Byron, Meg ;
Monks, Brian ;
Henry-Bezy, Meabh ;
Lawrence, Jeanne B. ;
O'Neill, Luke A. J. ;
Moore, Melissa J. ;
Caffrey, Daniel R. ;
Fitzgerald, Katherine A. .
SCIENCE, 2013, 341 (6147) :789-792
[4]   LncRNA TMPO-AS1 serves as a ceRNA to promote osteosarcoma tumorigenesis by regulating miR-199a-5p/WNT7B axis [J].
Cui, Huaan ;
Zhao, Jiang .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2020, 121 (03) :2284-2293
[5]   LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis [J].
Duan, Haixia ;
Li, Xiaojuan ;
Chen, Youyi ;
Wang, Yan ;
Li, Zhibin .
LIFE SCIENCES, 2019, 239
[6]   Long non-coding RNA HNF1A-AS1 mediated repression of miR-34a/SIRT1/p53 feedback loop promotes the metastatic progression of colon cancer by functioning as a competing endogenous RNA [J].
Fang, Changyi ;
Qiu, Shenglong ;
Sun, Feng ;
Li, Wei ;
Wang, Ziqi ;
Yue, Ben ;
Wu, Xuesong ;
Yan, Dongwang .
CANCER LETTERS, 2017, 410 :50-62
[7]   Metastatic Breast Cancer: Therapeutic Options According to Molecular Subtypes and Prior Adjuvant Therapy [J].
Guarneri, Valentina ;
Conte, PierFranco .
ONCOLOGIST, 2009, 14 (07) :645-656
[8]   Overexpression of AR-regulated lncRNA TMPO-AS1 correlates with tumor progression and poor prognosis in prostate cancer [J].
Huang, Wenhua ;
Su, Xinya ;
Yan, Wei ;
Kong, Zhe ;
Wang, Dan ;
Huang, Yan ;
Zhai, Qiaoli ;
Zhang, Xiaowei ;
Wu, Hai ;
Li, Yao ;
Li, Tao ;
Wan, Xuechao .
PROSTATE, 2018, 78 (16) :1248-1261
[9]   Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor [J].
Kalinichenko, VV ;
Major, ML ;
Wang, XH ;
Petrovic, V ;
Kuechle, J ;
Yoder, HM ;
Dennewitz, MB ;
Shin, B ;
Datta, A ;
Raychaudhuri, P ;
Costa, RH .
GENES & DEVELOPMENT, 2004, 18 (07) :830-850
[10]   FoxM1: At the crossroads of ageing and cancer [J].
Laoukili, Jamila ;
Stahl, Marie ;
Medema, Rene H. .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2007, 1775 (01) :92-102
123