Synthesis of multi-branched Au nanocomposites with distinct plasmon resonance in NIR-II window and controlled CRISPR-Cas9 delivery for synergistic gene-photothermal therapy

被引：38

作者：

Tao, Weiwei ^{[1
,2
,3
]}

Cheng, Xiaolan ^{[2
,3
]}

Sun, Dongdong ^{[2
,3
]}

Guo, Yang ^{[2
,3
]}

Wang, Neng ^{[1
]}

Ruan, Jie ^{[2
,3
]}

Hu, Yue ^{[2
,3
]}

Zhao, Min ^{[2
,3
]}

Zhao, Tong ^{[2
,3
]}

Feng, Hui ^{[2
,3
]}

Fan, Lu ^{[1
]}

Lu, Cai ^{[1
]}

Ma, Yong ^{[2
,3
]}

Duan, Jinao ^{[1
]}

Zhao, Ming ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Natl & Local Collaborat Engn Ctr Chinese Med Resou, Nanjing 210023, Peoples R China

[2] Nanjing Univ Chinese Med, Sch Chinese Med, Dept Integrated Chinese & Western Med, Nanjing 210023, Peoples R China

[3] Nanjing Univ Chinese Med, Sch Integrated Chinese & Western Med, Nanjing 210023, Peoples R China

来源：

BIOMATERIALS | 2022年 / 287卷

基金：

中国国家自然科学基金;

关键词：

Multi-branched Au; NIR-II; Photothermal therapy; CRISPR-Cas9; Thermoresistance; GOLD NANOPARTICLES; DRUG-DELIVERY; EXOCYTOSIS; IMPROVE; GROWTH;

D O I：

10.1016/j.biomaterials.2022.121621

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Clinical implementation of photothermal therapy (PTT) is mainly hampered by limited tissue penetration, undesirable thermal damage to normal tissues, and thermotolerence induced by heat shock proteins (HSPs). To overcome these obstacles, we constructed a novel gene-photothermal synergistic therapeutic nanoplatform composed of a multi-branched Au nanooctopus (AuNO) core and mesoporous polydopamine (mPDA) shell, followed by CRISPR-Cas9 ribonucleoprotein (RNP) loading and then polyethylene glycol-folic acid (PEG-FA) coating. AuNO was simply synthesized by adjusting the ratio of cetyltrimethylammonium chloride (CTAC) and cetyltrimethylammonium bromide (CTAB), which showed significant localized surface plasmon resonances in the NIR-II window, and exhibited an excellent tissue penetration capability and high photothermal conversion efficiency (PCE, 47.68%). Even, the PCE could be further increased to 66.17% by mPDA coating. Furthermore, the sequential modification of AuNO@mPDA using RNP and PEG-FA can down-regulate HSP90 alpha expression at tumor sites, enhance apoptosis and reduce the heat resistance of cancer cells. The synergistic effect of enhanced photothermal capacity and reduced thermoresistance addressed the multiple limitations of PTT, and presented excellent in vitro and in vivo antitumor efficacy, having great potential for the clinical application of PTT.

引用

页数：10

共 1 条

[1] A Dual-Enzyme-Responsive DNA-Based Nanoframework Enables Controlled Co-Delivery of CRISPR-Cas9 and Antisense Oligodeoxynucleotide for Synergistic Gene Therapy
Song, Nachuan
Chu, Yiwen
Li, Shuai
Fan, Xiaoting
Tang, Jianpu
Li, Hongjin
Tao, Ruoyu
Li, Fengqin
Li, Junru
Yang, Dayong
Liu, Peifeng
ADVANCED FUNCTIONAL MATERIALS, 2023, 33 (47)

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