Transcriptome profiling and digital gene expression analysis of sweet potato for the identification of putative genes involved in the defense response against Fusarium oxysporum f. sp batatas

被引:28
作者
Lin, Yuli [1 ,2 ,3 ]
Zou, Weikun [3 ]
Lin, Shiqiang [1 ,4 ]
Onofua, Dennis [3 ]
Yang, Zhijian [1 ,3 ]
Chen, Haizhou [3 ]
Wang, Songliang [3 ]
Chen, Xuanyang [1 ,2 ,3 ,5 ]
机构
[1] Fujian Prov Univ, Fujian Agr & Forestry Univ, Key Lab Crop Biotechnol, Fuzhou, Fujian, Peoples R China
[2] Minist Educ, Key Lab Genet Breeding & Multiple Applicat Crops, Fuzhou, Fujian, Peoples R China
[3] Fujian Agr & Forestry Univ, Dept Agron, Coll Crop Sci, Fuzhou, Fujian, Peoples R China
[4] Fujian Agr & Forestry Univ, Coll Life Sci, Dept Bioinformat, Fuzhou, Fujian, Peoples R China
[5] Fujian Prov Key Lab Crop Breeding Design, Fuzhou, Fujian, Peoples R China
来源
PLOS ONE | 2017年 / 12卷 / 11期
关键词
PATHOGENESIS-RELATED PROTEIN; ANTHOCYANIN ACCUMULATION; HETEROLOGOUS EXPRESSION; SALICYLIC-ACID; RESISTANCE; GENERATION; JASMONATE; PATHWAYS; DISEASE; IBMYB1A;
D O I
10.1371/journal.pone.0187838
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sweet potato production is constrained by Fusarium wilt, which is caused by Fusarium oxysporum f. sp. batatas (Fob). The identification of genes related to disease resistance and the underlying mechanisms will contribute to improving disease resistance via sweet potato breeding programs. In the present study, we performed de novo transcriptome assembly and digital gene expression (DGE) profiling of sweet potato challenged with Fob using Illumina HiSeq technology. In total, 89,944,188 clean reads were generated from 12 samples and assembled into 101,988 unigenes with an average length of 666 bp; of these unigenes, 62,605 (61.38%) were functionally annotated in the NCBI non-redundant protein database by BLASTX with a cutoff E-value of 10(-5). Clusters of Orthologous Groups (COG), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were examined to explore the unigenes' functions. We constructed four DGE libraries for the sweet potato cultivars JinShan57 (JS57, highly resistant) and XinZhongHua (XZH, highly susceptible), which were challenged with pathogenic Fob. Genes that were differentially expressed in the four libraries were identified by comparing the transcriptomes. Various genes that were differentially expressed during defense, including chitin elicitor receptor kinase 1 (CERK), mitogen-activated protein kinase (MAPK), WRKY, NAC, MYB, and ethylene-responsive transcription factor (ERF), as well as resistance genes, pathogenesisrelated genes, and genes involved in salicylic acid (SA) and jasmonic acid (JA) signaling pathways, were identified. These data represent a sequence resource for genetic and genomic studies of sweet potato that will enhance the understanding of the mechanism of disease resistance.
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页数:21
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