Determination of formaldehyde in blood plasma by high-performance liquid chromatography with fluorescence detection

被引:144
作者
Luo, WH
Li, H
Zhang, Y
Ang, CYW
机构
[1] Shantou Univ Med Coll, Cent Lab, Shantou 515031, Guangdong, Peoples R China
[2] US FDA, Natl Ctr Toxicol Res, US Dept HHS, Div Chem, Jefferson, AR 72079 USA
来源
JOURNAL OF CHROMATOGRAPHY B | 2001年 / 753卷 / 02期
关键词
formaldehyde;
D O I
10.1016/S0378-4347(00)00552-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An HPLC method was developed for the determination of formaldehyde in human blood plasma. The method was based on the determination of the fluorescent product of the chemical reaction between formaldehyde and ampicillin. A 0.2-ml aliquot of blood plasma was reacted directly with ampicillin under acidic and heating conditions. The reaction product was extracted from the matrix with diethyl ether and analyzed by reversed-phase HPLC with fluorescence detection. Recoveries of spiked formaldehyde at the low ppm (mug/ml) level were between 93% and 102% with relative standard deviations less than 8%. The limits of detection and quantitation of formaldehyde in blood plasma samples were 0.46 mug/ml and 0.87 mug/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:253 / 257
页数:5
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