Understanding the Role of Escherichia coli Hydrogenases and Formate Dehydrogenases in the FOF1-ATPase Activity during the Mixed Acid Fermentation of Mixture of Carbon Sources

被引:17
作者
Gevorgyan, Heghine [1 ]
Trchounian, Armen [1 ,2 ]
Trchounian, Karen [2 ]
机构
[1] Yerevan State Univ, Dept Biochem Microbiol & Biotechnol, Fac Biol, Yerevan, Armenia
[2] Yerevan State Univ, Sci Res Inst Biol, Fac Biol, 1 A Manoogian Str, Yerevan 0025, Armenia
关键词
Escherichia coli; formate dehydrogenases and hydrogenases; FOF1-ATPase; pH; potassium ions; ATP SYNTHASE; H-2; PRODUCTION; GLYCEROL FERMENTATION; ANAEROBIC CONDITIONS; EXTERNAL FORMATE; BATCH CULTURES; GAS-PRODUCTION; DIFFERENT PHS; HYF; F0F1-ATPASE;
D O I
10.1002/iub.1915
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During fermentation Escherichia coli produces di-hydrogen (H-2) via reversible membrane-bound [Ni-Fe]-hydrogenases (Hyd). This study describes the total and N,N-dicyclohexylcarbodiimide (DCCD) inhibited ATPase activity and H-2 production at various pHs in E. coli wild type and mutants encoding Hyd enzymes and formate dehydrogenases (FDH) on fermentation of glucose, glycerol, and formate. The highest total ATPase activity was detected at pH 7.5 in hyaB hybC selC (lacking large subunits of Hyd-1 and Hyd-2 and FDH, respectively) triple mutant. This ATPase activity was mainly due to the proton-translocating ATPase but in FDH mutant the DCCD inhibition was less compared to wild type. Potassium ions stimulated total ATPase activity at pH 5.5 (similar to)50% and 35% in wild type and hypF (lacking all Hyd enzymes) mutant, respectively. Moreover, K+ also stimulated DCCD inhibited ATPase activity similar to 1.7-fold-2-fold in strains where FDH was absent only at pH 5.5. DCCD inhibited H-2 production only at pH 5.5 in all assays. Taken together it is suggested that at low pH, FDH, and Hyd enzymes are linked with the FOF1-ATPase for regulating and maintaining the cytoplasmatic pH and thus proton motive force generation. FDH and Hyd enzymes have impact on the FOF1-ATPase activity depending on external pH and potassium ions. (c) 2018 IUBMB Life, 70(10):1040-1047, 2018
引用
收藏
页码:1040 / 1047
页数:8
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