Quantitative phase imaging by optimized asymmetric illumination

被引：3

作者：

Suzuki, Yoshimasa ^{[1
,2
]}

Odaira, Mayumi ^{[1
]}

Ohde, Hisashi ^{[1
]}

Kawata, Yoshimasa ^{[2
]}

机构：

[1] Olympus Corp, Opt Technol R&D Dept, Hachioji, Tokyo 1928512, Japan

[2] Shizuoka Univ, Grad Sch Sci & Technol, Hamamatsu, Shizuoka 4328561, Japan

来源：

APPLIED OPTICS | 2017年 / 56卷 / 25期

关键词：

HIGH-RESOLUTION; CONTRAST; MICROSCOPY; MORPHOLOGY;

D O I：

10.1364/AO.56.007237

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have presented a simple approach for quantitative phase imaging by optimizing asymmetric illumination of a conventional microscope. With this illumination, the light intensity modulation accompanying refraction at the surface profile of phase objects occurs, and "phase-gradient information" can be derived by detecting it. Two images with phase-gradient information on different axes are converted into the two-dimensional phase distribution of the specimen by introducing the phase-gradient transfer function, which is the intensity change due to refraction by the phase-gradient of a specimen. We experimentally confirm accurate and repeatable performance of our method and demonstrate phase imaging of live cells. (C) 2017 Optical Society of America

引用

页码：7237 / 7242

页数：6

共 17 条

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