Using micro-patterned sensors and cell self-assembly for measuring the oxygen consumption rate of single cells

被引：21

作者：

Etzkorn, James R. ^{[1
]}

Wu, Wen-Chung ^{[2
]}

Tian, Zhiyuan ^{[2
]}

Kim, Prince ^{[2
]}

Jang, Sei-Hum ^{[2
]}

Meldrum, Deirdre R. ^{[3
,4
]}

Jen, Alex K-Y ^{[2
]}

Parviz, Babak A. ^{[1
]}

机构：

[1] Univ Washington, Dept Elect Engn, Seattle, WA 98185 USA

[2] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98185 USA

[3] Arizona State Univ, Ira A Fulton Sch Engn, Tempe, AZ 85287 USA

[4] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA

来源：

JOURNAL OF MICROMECHANICS AND MICROENGINEERING | 2010年 / 20卷 / 09期

关键词：

GENE-EXPRESSION; HETEROGENEITY;

D O I：

10.1088/0960-1317/20/9/095017

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present a method for self-assembling arrays of live single cells on a glass chip using a photopatternable polymer to form micro-traps. We have studied the single-cell self-assembly method and optimized the process to obtain a 52% yield of single-trapped cells. We also report a method to measure the oxygen consumption rate of a single cell using micro-patterned sensors. These molecular oxygen sensors were fabricated around each micro-trap allowing optical interrogation of oxygen concentration in the immediate environment of the trapped cell. Micromachined micro-wells were then used to seal the trap, sensor and cell in order to determine the oxygen consumption rate of single cells. These techniques reported here add to the collection of tools for performing 'singe-cell' biology. An oxygen consumption rate of 1.05 +/- 0.28 fmol min(-1) was found for a data set consisting of 25 single A549 cells.

引用

页数：11

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