Effects of atrazine and chlorpyrifos on DNA methylation in the brain and gonad of the common carp

被引：41
作者：
Xing, Houjuan [1 ,2 ]
Wang, Chao [1 ]
Wu, Hongda [3 ]
Chen, Dechun [4 ]
Li, Shu [1 ,4 ]
Xu, Shiwen [4 ]
机构：
[1] Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China
[2] Anim Hlth Supervis Inst Heilongjiang Prov, Harbin 150069, Peoples R China
[3] Acad Agr Sci Heilongjiang Prov, Inst Anim Sci, Harbin 150086, Peoples R China
[4] Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2015年 / 168卷
关键词：
DNA methylation; Pesticides; mRNA; Westem blot; Fish; Principal component analysis; GENOME-WIDE; EXPOSURE; PESTICIDES; PROMOTER; FISH; HYPERMETHYLATION; EXPRESSION; WATER; GENOTOXICITY; ACTIVATION;
D O I：
10.1016/j.cbpc.2014.11.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA methylation is known to play an important role in the regulation of gene expression in animal. The purpose of the present study was to examine the effect of atrazine (ATR), chlorpyrifos (CPF) and combined ATR/CPF exposure on DNA methylation in the brain and gonad of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 40-d recovery to measure the levels of global DNA methylation and the expression of methylation enzymes (DNA methyltransferases (DNMTs) and methylcytosine binding domain 2 (MBD2)) in the brain and gonad tissues. The results revealed that a significant global DNA hypomethylation in the common carp exposed to ATR, CPF and their mixture was observed compared to the control fish. The MBD2 mRNA expression was up-regulated in the brain and gonad of the common carp exposed to ATR, CPF and their mixture, in contrast, the DNMTs mRNA expression was down-regulated. The information regarding the effects of ATR and CPF on DNA methylation status generated in this study is important for pesticides toxicology evaluation. However, the effect of ATR and CPF on the methylation status of specific genes, as well as its detailed mechanism requires further investigation. (C) 2014 Elsevier Inc. All rights reserved.
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页码：11 / 19
页数：9
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