Technical challenges of working with extracellular vesicles

被引：366

作者：

Ramirez, Marcel I. ^{[1
,2
]}

Amorim, Maria G. ^{[3
]}

Gadelha, Catarina ^{[4
]}

Milic, Ivana ^{[5
]}

Welsh, Joshua A. ^{[6
]}

Freitas, Vanessa M. ^{[7
]}

Nawaz, Muhammad ^{[7
,8
]}

Akbar, Naveed ^{[9
]}

Couch, Yvonne ^{[10
]}

Makin, Laura ^{[11
]}

Cooke, Fiona ^{[12
]}

Vettore, Andre L. ^{[13
]}

Batista, Patricia X. ^{[13
]}

Freezor, Roberta ^{[14
]}

Pezuk, Julia A. ^{[15
]}

Rosa-Fernandes, Livia ^{[7
,16
]}

Carreira, Ana Claudia O. ^{[7
]}

Devitt, Andrew ^{[5
]}

Jacobs, Laura ^{[17
]}

Silva, Israel T. ^{[3
]}

Coakley, Gillian ^{[18
]}

Nunes, Diana N. ^{[3
]}

Carter, Dave ^{[19
]}

Palmisano, Giuseppe ^{[7
,20
]}

Dias-Neto, Emmanuel ^{[3
,7
]}

机构：

[1] Fundacao Oswaldo Cruz, Rio De Janeiro, RJ, Brazil

[2] Univ Fed Parana, Curitiba, Parana, Brazil

[3] AC Camargo Canc Ctr, CIPE, Sao Paulo, SP, Brazil

[4] Univ Nottingham, Sch Life Sci, Nottingham, England

[5] Aston Univ, Sch Life & Hlth Sci, Birmingham, W Midlands, England

[6] Univ Southampton, Southampton, England

[7] Univ Sao Paulo, Sao Paulo, SP, Brazil

[8] Univ Gothenburg, Gothenburg, Sweden

[9] Univ Oxford, Div Cardiovasc Med, Oxford, England

[10] Univ Oxford, Acute Stroke Programme, RDM Investigat Med, Oxford, England

[11] Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England

[12] Univ St Andrews, St Andrews, Fife, Scotland

[13] Univ Fed Sao Paulo, Campus Diadema, Diadema, Brazil

[14] London Metropolitan Univ, London, England

[15] Univ Anhanguera Sao Paulo, Sao Paulo, Brazil

[16] Univ Southern Denmark, Odense, Denmark

[17] Tech Univ Munich, Munich, Germany

[18] Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow, Lanark, Scotland

[19] Oxford Brookes Univ, Oxford, England

[20] Fdn Santa Lucia, IRCCS, Rome, Italy

来源：

NANOSCALE | 2018年 / 10卷 / 03期

基金：

巴西圣保罗研究基金会; 英国生物技术与生命科学研究理事会;

关键词：

CELL-DERIVED EXOSOMES; PROTEIN IDENTIFICATION TECHNOLOGY; TRANSMISSION ELECTRON-MICROSCOPY; PROTEOMIC ANALYSIS; PROSTATE-CANCER; LIPID EXTRACTION; PREANALYTICAL PARAMETERS; SIMULTANEOUS ENRICHMENT; MICROFLUIDIC DEVICE; MEMBRANE-VESICLES;

D O I：

10.1039/c7nr08360b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

引用

页码：881 / 906

页数：26

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