Improving the quality of industrially important enzymes by directed evolution

被引：32

作者：

Chirumamilla, RR ^{[1
]}

Muralidhar, R ^{[1
]}

Marchant, R ^{[1
]}

Nigam, P ^{[1
]}

机构：

[1] Univ Ulster, Biotechnol Res Grp, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2001年 / 224卷 / 1-2期

关键词：

directed evolution; substrate specificity; activity; enantioselectivity; thermal stability; mutagenesis;

D O I：

10.1023/A:1011904405002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Directed evolution is a new process for developing industrially viable biocatalysts. This technique does not require a comprehensive knowledge of the relationships between sequence structure and function of proteins as required by protein engineering. It mimics the process of Darwinian evolution in a test tube combining random mutagenesis and recombination with screening or selection for enzyme variants that have the desired properties. Directed evolution helps in enhancing the enzyme performance both in natural and synthetic environments. This article reviews the process of directed evolution and its application to improve substrate specificity, activity, enantioselectivity and thermal stability.

引用

页码：159 / 168

页数：10

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