Exposure of rhesus monkeys to cowpox virus Brighton Red by large-particle aerosol droplets results in an upper respiratory tract disease

被引：4

作者：

Johnson, Reed F. ^{[1
]}

Hammoud, Dima A. ^{[2
]}

Perry, Donna L. ^{[3
]}

Solomon, Jeffrey ^{[4
]}

Moore, Ian N. ^{[5
]}

Lackemeyer, Matthew G. ^{[3
]}

Bohannon, Jordan K. ^{[3
]}

Sayre, Philip J. ^{[3
]}

Minai, Mahnaz ^{[5
]}

Papaneri, Amy B. ^{[1
]}

Hagen, Katie R. ^{[3
]}

Janosko, Krisztina B. ^{[3
]}

Jett, Catherine ^{[3
]}

Cooper, Kurt ^{[3
]}

Blaney, Joseph E. ^{[6
]}

Jahrling, Peter B. ^{[1
,3
]}

机构：

[1] NIAID, Emerging Viral Pathogens Sect, NIH, Frederick, MD 21702 USA

[2] NIH, Ctr Infect Dis Imaging Radiol & Imaging Sci, Ctr Clin, Bethesda, MD 20892 USA

[3] NIAID, Integrated Res Facil, NIH, Frederick, MD 21702 USA

[4] Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Clin Res Directorate, Clin Monitoring Res Program, Frederick, MD 21702 USA

[5] NIAID, Infect Dis Pathogenesis Sect, NIH, Bethesda, MD 20892 USA

[6] NIAID, Off Sci Director, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF GENERAL VIROLOGY | 2016年 / 97卷

基金：

美国国家卫生研究院;

关键词：

CYNOMOLGUS MACAQUES; ECTROMELIA VIRUS; SMALLPOX VIRUS; INFECTION; IDENTIFICATION; INOCULATION; OUTBREAK; PROTEIN; LAVAGE; MODEL;

D O I：

10.1099/jgv.0.000501

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We previously demonstrated that small-particle (0.5-3.0 mu m) aerosol infection of rhesus monkeys (Macaca mulatta) with cowpox virus (CPXV)-Brighton Red (BR) results in fulminant respiratory tract disease characterized by severe lung parenchymal pathology but only limited systemic virus dissemination and limited classic epidermal pox-like lesion development (Johnson et al., 2015). Based on these results, and to further develop CPXV as an improved model of human smallpox, we evaluated a novel large-particle aerosol (7.0-9.0 mu m) exposure of rhesus monkeys to CPXV-BR and monitored for respiratory tract disease by serial computed tomography (CT). As expected, the upper respiratory tract and large airways were the major sites of virus-induced pathology following large-particle aerosol exposure. Large-particle aerosol CPXV exposure of rhesus macaques resulted in severe upper airway and large airway pathology with limited systemic dissemination.

引用

页码：1942 / 1954

页数：13

共 2 条

[1] Small particle aerosol inoculation of cowpox Brighton Red in rhesus monkeys results in a severe respiratory disease
Johnson, Reed E.
Hammoud, Dima A.
Lackemeyer, Matthew G.
Yellayi, Srikanth
Solomon, Jeffrey
Bohannon, Jordan K.
Janosko, Krisztina B.
Jett, Catherine
Cooper, Kurt
Blaney, Joseph E.
Jahrling, Peter B.
VIROLOGY, 2015, 481 : 124 - 135
[2] The Use of Large-Particle Aerosol Exposure to Nipah Virus to Mimic Human Neurological Disease Manifestations in the African Green Monkey
Lee, Ji Hyun
Hammoud, Dima A.
Cong, Yu
Huzella, Louis M.
Castro, Marcelo A.
Solomon, Jeffrey
Laux, Joseph
Lackemeyer, Matthew
Bohannon, J. Kyle
Rojas, Oscar
Byrum, Russ
Adams, Ricky
Ragland, Danny
St Claire, Marisa
Munster, Vincent
Holbrook, Michael R.
JOURNAL OF INFECTIOUS DISEASES, 2020, 221 : S419 - S430

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