High-Throughput Screening for Inhibitors of Sialyl- and Fucosyltransferases

被引：50
|
作者：
Rillahan, Cory D. [1 ]
Brown, Steven J. [1 ]
Register, Amy C. [1 ]
Rosen, Hugh [1 ]
Paulson, James C. [1 ]
机构：
[1] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 52期
关键词：
carbohydrates; fucose; glycoproteins; glycosyltransferases; sialic acids; GDP-L-FUCOSE; ASSAY;
D O I：
10.1002/anie.201105065
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Sweet screens: A high-throughput screening platform for identification of inhibitors of sialyl- and fucosyltransferases based on fluorescence polarization (FP) has been developed. An analogue of the natural donor substrate carrying a fluorescent label (green star) is transferred to a glycoprotein acceptor, which results in robust FP (see picture). The screening of 16 000 compounds against different glycosyltransferases has identified various interesting inhibitors. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：12534 / 12537
页数：4
相关论文
共 50 条
  • [41] Identification of avermectin-high-producing strains by high-throughput screening methods
    Gao, Hong
    Liu, Mei
    Zhou, Xianlong
    Liu, Jintao
    Zhuo, Ying
    Gou, Zhongxuan
    Xu, Bing
    Zhang, Wenquan
    Liu, Xiangyang
    Luo, Aiqun
    Zheng, Chuansen
    Chen, Xiaoping
    Zhang, Lixin
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) : 1219 - 1225
  • [42] Screening Europe 2010: an update about the latest technologies and applications in high-throughput screening
    Merten, Christoph A.
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2010, 10 (05) : 559 - 563
  • [43] High-Throughput Strategy to Identify Inhibitors of Histone-Binding Domains
    Wagner, Elise K.
    Albaugh, Brittany N.
    Denu, John M.
    NUCLESOMES, HISTONES & CHROMATIN, PT A, 2012, 512 : 161 - 185
  • [44] A high-throughput whole cell screen to identify inhibitors of Mycobacterium tuberculosis
    Ollinger, Juliane
    Kumar, Anuradha
    Roberts, David M.
    Bailey, Mai A.
    Casey, Allen
    Parish, Tanya
    PLOS ONE, 2019, 14 (01):
  • [45] MALDI-TOF Mass Spectrometry-Based High-Throughput Screening for Inhibitors of the Cytosolic DNA Sensor cGAS
    Simon, Roman P.
    Winter, Martin
    Kleiner, Carola
    Ries, Robert
    Schnapp, Gisela
    Heimann, Annekatrin
    Li, Jun
    Zuvela-Jelaska, Ljiljana
    Bretschneider, Tom
    Luippold, Andreas H.
    Reindl, Wolfgang
    Bischoff, Daniel
    Buettner, Frank H.
    SLAS DISCOVERY, 2020, 25 (04) : 372 - 383
  • [46] Identification of Small-Molecule Inhibitors of Neutral Ceramidase (nCDase) via Target-Based High-Throughput Screening
    Otsuka, Yuka
    Airola, Michael, V
    Choi, Yong-Mi
    Coant, Nicolas
    Snider, Justin
    Cariello, Chris
    Saied, Essa M.
    Arenz, Christoph
    Bannister, Thomas
    Rahaim, Ron, Jr.
    Hannun, Yusuf A.
    Shumate, Justin
    Scampavia, Louis
    Haley, John D.
    Spicer, Timothy P.
    SLAS DISCOVERY, 2021, 26 (01) : 113 - 121
  • [47] High-throughput platforms for the screening of new therapeutic targets for neurodegenerative diseases
    Rocha, Daniela N.
    Carvalho, Eva D.
    Pego, Ana Paula
    DRUG DISCOVERY TODAY, 2016, 21 (09) : 1355 - 1366
  • [48] Conjugated Polythiophene for Rapid, Simple, and High-Throughput Screening of Antimicrobial Photosensitizers
    Li, Ruihua
    Niu, Ruimin
    Qi, Junjie
    Yuan, Hongbo
    Fan, Yibing
    An, Hailong
    Yan, Wenmin
    Li, Huanrong
    Zhan, Yong
    Xing, Chengfen
    ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (27) : 14569 - 14572
  • [49] Acanthamoeba castellanii: A new high-throughput method for drug screening in vitro
    Ortega-Rivas, Antonio
    Padron, Jose M.
    Valladares, Basilio
    Elsheikha, Hany M.
    ACTA TROPICA, 2016, 164 : 95 - 99
  • [50] Metal Impurities Cause False Positives in High-Throughput Screening Campaigns
    Hermann, Johannes C.
    Chen, Yingsi
    Wartchow, Charles
    Menke, John
    Gao, Lin
    Gleason, Shelley K.
    Haynes, Nancy-Ellen
    Scott, Nathan
    Petersen, Ann
    Gabriel, Stephen
    Binh Vu
    George, Kelly M.
    Narayanan, Arjun
    Li, Shirley H.
    Qian, Hong
    Beatini, Nanda
    Niu, Linghao
    Gan, Qing-Fen
    ACS MEDICINAL CHEMISTRY LETTERS, 2013, 4 (02): : 197 - 200
12345