High-Throughput Screening for Inhibitors of Sialyl- and Fucosyltransferases

被引：50

作者：

Rillahan, Cory D. ^{[1
]}

Brown, Steven J. ^{[1
]}

Rosen, Hugh ^{[1
]}

Paulson, James C. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 52期

关键词：

carbohydrates; fucose; glycoproteins; glycosyltransferases; sialic acids; GDP-L-FUCOSE; ASSAY;

D O I：

10.1002/anie.201105065

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sweet screens: A high-throughput screening platform for identification of inhibitors of sialyl- and fucosyltransferases based on fluorescence polarization (FP) has been developed. An analogue of the natural donor substrate carrying a fluorescent label (green star) is transferred to a glycoprotein acceptor, which results in robust FP (see picture). The screening of 16 000 compounds against different glycosyltransferases has identified various interesting inhibitors. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：12534 / 12537

页数：4

共 50 条

[31] A high-throughput target-based screening approach for the identification and assessment of Mycobacterium tuberculosis mycothione reductase inhibitors
Smiejkowska, Natalia
Oorts, Lauren
Van Calster, Kevin
De Vooght, Linda
Geens, Rob
Mattelaer, Henri-Philippe
Augustyns, Koen
Strelkov, Sergei V.
Lamprecht, Dirk
Temmerman, Koen
Sterckx, Yann G. -J.
Cappoen, Davie
Cos, Paul
MICROBIOLOGY SPECTRUM, 2024, 12 (03):
[32] High-throughput cell-based screening using scintillation proximity assay for the discovery of inositol phosphatase inhibitors
Zheng, W
Brandish, PE
Kolodin, DG
Scolnick, EM
Strulovici, B
JOURNAL OF BIOMOLECULAR SCREENING, 2004, 9 (02) : 132 - 140
[33] High-Throughput Identification of Promiscuous Inhibitors from Screening Libraries with the Use of a Thiol-Containing Fluorescent Probe
McCallum, Megan M.
Nandhikonda, Premchendar
Temmer, Jonathan J.
Eyermann, Charles
Simeonov, Anton
Jadhav, Ajit
Yasgar, Adam
Maloney, David
Arnold, Alexander
JOURNAL OF BIOMOLECULAR SCREENING, 2013, 18 (06) : 705 - 713
[34] Non-stoichiometric inhibition in biochemical high-throughput screening
Coan, Kristin E. D.
Ottl, Johannes
Klumpp, Martin
EXPERT OPINION ON DRUG DISCOVERY, 2011, 6 (04) : 405 - 417
[35] New Device for High-Throughput Viability Screening of Flow Biofilms
Benoit, Michael R.
Conant, Carolyn G.
Ionescu-Zanetti, Cristian
Schwartz, Michael
Matin, A.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (13) : 4136 - 4142
[36] Design and Application of a High-Throughput, High-Content Screening System for Natural Product Inhibitors of the Human Parasite Trichomonas vaginalis
King, Jarrod B.
Carter, Adam C.
Dai, Wentao
Lee, Jin Woo
Kil, Yun-Seo
Du, Lin
Helff, Sara K.
Cai, Shengxin
Huddle, Brandt C.
Cichewicz, Robert H.
ACS INFECTIOUS DISEASES, 2019, 5 (08): : 1456 - 1470
[37] Identification of Angiogenesis Inhibitors Using a Co-culture Cell Model in a High-Content and High-Throughput Screening Platform
Li, Shuaizhang
Hsu, Chia-Wen
Sakamuru, Srilatha
Zou, Chaozhong
Huang, Ruili
Xia, Menghang
SLAS TECHNOLOGY, 2018, 23 (03): : 217 - 225
[38] Fluorescent probe for high-throughput screening of membrane protein expression
Backmark, A. E.
Olivier, N.
Snijder, A.
Gordon, E.
Dekker, N.
Ferguson, A. D.
PROTEIN SCIENCE, 2013, 22 (08) : 1124 - 1132
[39] Gaussia Luciferase Variant for High-Throughput Functional Screening Applications
Maguire, Casey A.
Deliolanis, Nikolaos C.
Pike, Lisa
Niers, Johanna M.
Tjon-Kon-Fat, Lee-Ann
Sena-Esteves, Miguel
Tannous, Bakhos A.
ANALYTICAL CHEMISTRY, 2009, 81 (16) : 7102 - 7106
[40] The Application of Phenotypic High-Throughput Screening Techniques to Cardiovascular Research
Etzion, Yoram
Muslin, Anthony J.
TRENDS IN CARDIOVASCULAR MEDICINE, 2009, 19 (06) : 207 - 212

← 1 2 3 4 5 →