EFFECTS OF THE ORAL ADMINISTRATION OF BIFIDOBACTERIUM LACTIS AD011 AND LACTOBACILLUS ACIDOPHILUS ADO31 ON MICE WITH OVALBUMIN-INDUCED FOOD ALLERGY

Objective: To explore the influences of the oral administration of lactate Bifidobacterium AD011 and Lactobacillus acidophilus AD031 on mice models with food allergy induced by ovalbumin. Methods: Sixty healthy male BALB/c mice were selected and divided into the allergic model group (n=15), low dosage treatment group (n=15), high dosage treatment group (n=15) and the control group (n=15) using random number methods. The allergy model group was given an intraperitoneal injection of 1 mg aluminum solution and 10 mu g OVA normal saline, and a food allergy model was established. Mice in the low dosage treatment group were given infra-gastric administration of lactate Bifidobacterium AD011 and Lactobacillus acidophilus AD031 within one hour after the establishment of the food allergy model (each 1x108 CFU/mL, 100 mu L/d). In the high dosage treatment group, mice models were formed according to the above methods and given an intro-gastric administration of lactate Bifidobacterium ADO11 and Lactobacillus acidophilus AD031 within one hour (each I x 109 CFU/mL, 100 mu L/d). The control group was given an intro-gastric administration of normal saline equal to the volume of that of the allergy model group. Allergic symptoms, microstructure of the intestinal tissue, spleen, thymus gland tissue, body mass, levels of IgE, IL-4 and IFN-gamma, and Escherichia coli and Bacteroides levels were observed in the various groups. Results: Allergy symptom scores, IL-4 level, and Escherichia coil and Bacteroides levels in the allergic mice model were obviously higher than those of the control group and the treatment group, with statistical significance (P<0.05); allergy symptom scores, IL-4 level, and Escherichia coli level in the high dosage group were obviously lower than those of the low dosage group, with statistical significance (P<0.05). The body mass, IgE level, ratio of IFN-gamma/IL-4 and IFN-gamma level in the allergic mice model group were obviously lower than those of the control group and the treatment group, with statistical significance (P<0.05). The IgE level, ratio of IFN-gamma/IL-4 and IFN-gamma levels in the high dosage group were obviously higher than those of the low dosage treatment group, with statistical significance (P<0.05). The differences between thymus index and spleen index of mice in four groups showed no statistical significance (P>0.05); the intestinal mucous membrane of mice in the control group and the treatment group was intact. Small intestinal cells had a close arrangement; the intestinal mucous membrane of mice in the allergic mice model was necrotic. Some epithelial cells showed edema and even necrosis. Conclusion: Lactate Bifidobacterium AD011 and Lactobacillus acidophilus AD031 can improve allergic symptoms in mice with . food allergy induced by OVA, relieve inflammation of small intestinal tissue, increase body mass and serum IgE and IFN-gamma levels, and lower IL-4, Escherichia coli and Bacteroides levels.