Purification and characterization of an acid protease produced by a mutant strain of Aspergillus niger

被引：0

作者：

Dasgupta, Dolanchanpa ^{[1
]}

Ganguly, Subhadeep ^{[1
]}

Bnnik, Ajit Kumar ^{[1
]}

机构：

[1] Univ Calcutta, Dept Chem Engn, Div Biochem Engn, Kolkata 700009, India

来源：

JOURNAL OF THE INDIAN CHEMICAL SOCIETY | 2011年 / 88卷 / 09期

关键词：

Acid protease; mutant strain; Aspergillus niger; EXTRACELLULAR PROTEASE; PROTEINASE;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Aspergillus niger, isolated from the soil, secretes an acidic protease into the medium on exposure to UV-irradiation for 1, 2; 3, h, the organism was Mutated and the mutants thus obtained were screened for maximum protease production. This extracellular protease was purified to apparent homogeneity by chromatography on DEAE-cellulose. Homogeneity was ascribed by electrophoresis. The molecular weight, as determined by SDS-PAGE was 39800 daltons. This protease has an optimum activity at a pH of 6.0 and temperature of 37 C. It is inhibited by metal ions like Cu2+ but is active in the presence of metal ions like Mn2+ and Mg2+.

引用

页码：1407 / 1411

页数：5

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