System maps for retention of small neutral compounds on a superficially porous particle column in reversed-phase liquid chromatography

被引：19

作者：

Atapattu, Sanka N. ^{[1
]}

Poole, Colin F. ^{[2
]}

Praseuth, Mike B. ^{[3
]}

机构：

[1] CanAm Biores Inc, Winnipeg, MB R3T 0P4, Canada

[2] Wayne State Univ, Dept Chem, Rm 185 Chem, Detroit, MI 48202 USA

[3] Phenomenex Inc, Torrance, CA 90501 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2016年 / 1468卷

关键词：

Reversed-phase liquid chromatography; Retention; Solvation parameter model; System maps; Superficially porous particles; Column characterization; SILICA STATIONARY-PHASE; SOLVATION ENERGY RELATIONSHIPS; OPEN-TUBULAR COLUMNS; SEPARATION CHARACTERISTICS; SOLUTE DESCRIPTORS; SELECTIVITY; HPLC; RP; INSIGHTS; MODEL;

D O I：

10.1016/j.chroma.2016.09.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The system constants of the solvation parameter model are used to prepare system maps for the retention of small neutral molecules on the ocadecylsiloxane-bonded silica superficially porous particle stationary phase (Kinetex C18) for aqueous-organic solvent mobile phases containing 10-70% (v/v) methanol or acetonitrile. A comparison of the system constants with eight commercially available octadecylsiloxane-bonded silica columns for the same separation conditions confirms that the general retention properties of Kinetex C-18 are similar to totally porous octadecylsiloxane-bonded silica stationary phases and that method transfer should be no more difficult than that usually observed when substituting one octadecylsiloxane-bonded silica column for another. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：250 / 256

页数：7

共 46 条

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