Isolation of Trametes hirsuta La-7 with high laccase-productivity and its application in metabolism of 17β-estradiol

被引：40

作者：

Sun, Kai ^{[1
]}

Cheng, Xing ^{[1
]}

Yu, Jialin ^{[1
]}

Chen, Luojian ^{[1
]}

Wei, Jiajun ^{[1
]}

Chen, Wenjun ^{[1
]}

Wang, Jun ^{[1
]}

Li, Shunyao ^{[2
]}

Liu, Qingzhu ^{[1
]}

Si, Youbin ^{[1
]}

机构：

[1] Anhui Agr Univ, Sch Resources & Environm, Anhui Prov Key Lab Farmland Ecol Conservat & Poll, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

[2] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2020年 / 263卷

基金：

美国国家科学基金会;

关键词：

Trametes hirsuta; Laccase; 17; beta-estradiol; HRMS combined With C-13-labeling; Metabolic pathway; EMERGING CONTAMINANTS; ENDOCRINE DISRUPTORS; ASSISTED DEGRADATION; PLEUROTUS-OSTREATUS; BISPHENOL-A; PURIFICATION; CATALYSIS; REMOVAL;

D O I：

10.1016/j.envpol.2020.114381

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Estrogens, which are extensive in the eco-environments, are a category of high-toxic emerging contaminants that induce metabolic disorders and even carcinogenic risks in wildlife and humans. Here we investigate whether fungus-secreted laccase can be used as a green catalyst to eliminate a representative estrogen, 17 beta-estradiol (E2). A white-rot fungus Trametes hirsuta La-7 with high laccase-productivity, was isolated from pig manure-contaminated soil. Extracellular laccase activity expressed by strain La-7 was 65.4 U.mL(-1) for a 3 d inoculation under the optimal fermentation parameters. The concentrated-crude laccase from Trametes hirsuta La-7 (CC-ThLac) was capable of effectively metabolizing E2 at pH 4-6, and the apparent pseudo first-order reaction rate constant and half-life values were respectively 0.027-0.055 min(-1) and 25.86-12.67 min (R-2 > 0.98). The mass measurement of high-resolution mass spectrometry in combination with C-13-isotope labeling identified that the main by-products of E2 metabolism were dimers, trimers, and tetramers, which are consistent with radical-driven C-C and/or C-O-C covalent coupling pathway, involving the initial enzymatic production of phenoxy radical intermediates and then the successive oxidative-oligomerization of radical intermediates. The formation of oligomers dramatically reduced the estrogenic activity of E2. Additionally, CC-ThLac also exhibited high-efficiency metabolism capability toward E2 in the natural water and pig manure, with more than 94.4% and 91.0% of E2 having been metabolized, respectively. These findings provide a broad prospect for the clean biotechnological applications of Trametes hirsuta La-7 in estrogen-contaminated ecosystems. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：11