Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Simultaneous Detection of Stimulants and Diuretics in Urine

被引：24
作者：
Hsu, Ku Fu [2 ]
Chien, Kuei-Yu [3 ]
Chang-Chien, Guo Ping [4 ]
Lin, Su Fan [4 ]
Hsu, Pei Hsuan [4 ]
Hsu, Mei-Chich [1 ]
机构：
[1] Natl Taiwan Sport Univ, Grad Inst Sports Sci, Kweishan 33301, Taoyuan County, Taiwan
[2] Natl Taiwan Sport Univ, Grad Inst Phys Educ, Kweishan 33301, Taoyuan County, Taiwan
[3] Kainan Univ, Dept Geriatr Hlth Promot, Luzhu Shiang 33857, Taoyuan County, Taiwan
[4] ChengShiu Univ, Super Micro Mass Res & Technol Ctr, Kaohsiung 833, Niaosong County, Taiwan
来源：
JOURNAL OF ANALYTICAL TOXICOLOGY | 2011年 / 35卷 / 09期
关键词：
SOLID-PHASE EXTRACTION; GAS-CHROMATOGRAPHY; DOPING ANALYSIS; AGENTS; GLUCOCORTICOIDS; AMPHETAMINE; DRUGS;
D O I：
10.1093/anatox/35.9.665
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This study established a simultaneous screening method based on solid-phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS-MS) for the detection of 23 stimulants and 23 diuretics in human urine. An electrospray ionization source and multiple reaction monitoring were used for data acquisition. All stimulants and diuretics were separated in less than 12.52 min. The limits of detection were in the range of 25-500 ng/mL for stimulants and 25-125 ng/mL for diuretics. To evaluate the performance of this method, urine samples were collected from 1627 athletes in Taiwan, and 7 positive samples were found. This LC-MS-MS method not only meets the minimum required performance limits set by theWorld Anti-Doping Agency but also provides a fast way to analyze the authentic urine samples in doping control laboratories.
引用
收藏
页码：665 / 674
页数：10
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