Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin

The effect of homogeneous fibrin (Fb), collagen (Coll) and composite fibrin-heparin (Fb-Hp), fibrin-collagen (Fb-Coll) membranes on in vitro release of platelet-derived growth factor (PDGF-BB) was evaluated in the presence or absence of amoxicillin using of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 272 urn. The process of the PDGF-BB growth factor and amoxicillin release from the studied membranes was of a two-phase nature in the majority of the systems analysed. The PDGF-BB was released in the highest amount from the Coll membrane (M7) without the presence of amoxicillin -546.2 +/- 7.47 pg, t(0.5) = 0.88 h and 202.5 +/- 6.83 pg, t(0.5) = 26.65 h during the first phase and second phase, respectively. The lowest PDGF-BB release was observed from composite M4 (Fb-Hp) membrane -5.88 +/- 0.81 pg. t(0.5) = 1.69 h; and 110.2 +/- 6.48 pg, t(0.5) = 855.6 h during first and second phase respectively. An optimal release of amoxicillin was observed in the case of the composite M6 (Pb-Coll) membrane - only in the second phase: 64.2 +/- 7.8 rig, t(0.5) = 83.5 h. The lowest and delayed amoxicillin release was achieved for M4 membrane (approx. 17.1 +/- 1.12 mu g, t(0.5) = 46.5 h). The results of the PDGF-BB release and amoxicillin from membranes indicated a correlation between the level of release and composition of the film. Our results suggested that fibrin and collagen membranes may be beneficial to enhance periodontal bone regeneration.