Quantitative detection of metallo-β-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection

被引：7

作者：

Motoshima, Maiko ^{[1
,3
]}

Yanagihara, Katsunori ^{[1
,3
]}

Yamamoto, Kazuko ^{[2
,3
]}

Morinaga, Yoshitomo ^{[1
,3
]}

Matsuda, Junichi ^{[1
,3
]}

Sugahara, Kazuyuki ^{[1
,3
]}

Hirakata, Yoichi ^{[1
,3
]}

Yamada, Yasuaki ^{[1
,3
]}

Kohno, Shigeru ^{[2
,3
]}

Kamihira, Shimeru ^{[1
,3
]}

机构：

[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan

[2] Nagasaki Univ, Dept Internal Med 2, Grad Sch Biomed Sci, Nagasaki 8528501, Japan

[3] Nagasaki Univ Hosp, Cent Diagnost Lab, Nagasaki 8528501, Japan

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 02期

关键词：

Pseudomonas aeruginosa; quantitative detection; metallo-beta-lactamase; melting curve analysis; blaIMP; gyrB;

D O I：

10.1016/j.diagmicrobio.2008.01.018

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：222 / 226

页数：5