Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins

被引：51
作者：
Chang, MS
Huang, CJ
Chen, ML
Chen, ST
Fan, CC
Chu, JM
Lin, WC
Yang, YC
机构：
[1] Mackay Mem Hosp, Dept Med Res, Taipei, Taiwan
[2] Mackay Mem Hosp, Dept Pathol, Taipei, Taiwan
[3] Mackay Mem Hosp, Dept Obstet & Gynecol, Taipei, Taiwan
[4] Acad Sinica, Inst Biol Chem, Taipei, Taiwan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 287卷 / 01期
关键词：
hMAP126; mitotic spindle; p29;
D O I：
10.1006/bbrc.2001.5554
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
One novel gene product, hMAP126, was demonstrated to interact with p29 in the yeast two-hybrid assay. The full-length cDNA of hMAP126 has been obtained and encodes a protein of 1120 amino acids. Multiple tissue Northern blot analysis showed that hMAP126 was abundantly expressed in the testis. Polyclonal antiserum against hMAP126 was raised and affinity-purification of anti-hMAP126 antibodies was performed. The subcellular distribution of hMAP126 was localized to the mitotic spindle. Furthermore, hMAP126 was identified to be post-translationally modified and phosphorylated by p34(cdc2) kinase in vitro. Taken together, we have isolated a novel protein, hMAP126, which may be involved in the functional and dynamic regulation of mitotic spindles. (C) 2001 Academic Press.
引用
收藏
页码：116 / 121
页数：6
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