Long non-coding RNA DBH-AS1 promotes cancer progression in diffuse large B-cell lymphoma by targeting FN1 via RNA-binding protein BUD13

被引：37
作者：
Song, Yanping [1 ]
Gao, Feng [2 ]
Peng, Yi [3 ]
Yang, Xuejie [4 ]
机构：
[1] First Hosp Jilin Univ, Dept Oncol, 71 Xinmin St, Changchun 130000, Jilin, Peoples R China
[2] First Hosp Jilin Univ, Hematol Canc Ctr, 71 Xinmin St, Changchun 130000, Jilin, Peoples R China
[3] First Hosp Jilin Univ, Dept Hematol, 71 Xinmin St, Changchun 130021, Jilin, Peoples R China
[4] Inner Mongolia BAOTOU Canc Hosp, Lymphomammary Internal Med, 18 Tuanjie St, Baotou 014030, Inner Mongolia, Peoples R China
来源：
CELL BIOLOGY INTERNATIONAL | 2020年 / 44卷 / 06期
关键词：
BUD13; DBH-AS1; diffuse large B-cell lymphoma; FN1; UP-REGULATION; PROLIFERATION; EXPRESSION; CARCINOMA; PREDICTS;
D O I：
10.1002/cbin.11327
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Diffuse large B-cell lymphoma (DLBC) is a subtype of lymphoma with the worst prognosis. Existing treatment methods are not effective enough due to its high occurrence of metastasis. Therefore, identification of effective therapeutic targets is becoming increasingly important. In this research, long non-coding RNA dopamine beta hydroxylase antisense RNA 1 (DBH-AS1) was found to be upregulated in DLBC tissues and cells. Knockdown of DBH-AS1 suppressed the proliferation, migration, and invasion of cancer cells. Afterwards, RNA-binding protein BUD13 homolog (BUD13) was found to be upregulated in cancer tissues and cells while binding to DBH-AS1. Fibronectin 1 (FN1) was the downstream messenger RNA (mRNA) of BUD13. FN1 was upregulated in DLBC and was positively correlated with DBH-AS1. Further rescue assays proved that DBH-AS1 mediated FN1 expression by recruiting BUD13. In the meantime, BUD13 stabilized FN1 mRNA to promote FN1 expression. In this way, DBH-AS1/BUD13/FN1 axis was confirmed. A set of rescue assays proved that DBH-AS1 regulated DLBC progression via BUD13 and FN1. The function and mechanism of DBH-AS1 were investigated for the first time in DLBC. DBH-AS1 might become a therapeutic target in lymphoma treatment in the future.
引用
收藏
页码：1331 / 1340
页数：10
相关论文
共 28 条
[1]   Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling [J].
Alizadeh, AA ;
Eisen, MB ;
Davis, RE ;
Ma, C ;
Lossos, IS ;
Rosenwald, A ;
Boldrick, JG ;
Sabet, H ;
Tran, T ;
Yu, X ;
Powell, JI ;
Yang, LM ;
Marti, GE ;
Moore, T ;
Hudson, J ;
Lu, LS ;
Lewis, DB ;
Tibshirani, R ;
Sherlock, G ;
Chan, WC ;
Greiner, TC ;
Weisenburger, DD ;
Armitage, JO ;
Warnke, R ;
Levy, R ;
Wilson, W ;
Grever, MR ;
Byrd, JC ;
Botstein, D ;
Brown, PO ;
Staudt, LM .
NATURE, 2000, 403 (6769) :503-511
[2]   Non-Hodgkin lymphoma: Diagnosis and treatment [J].
Ansell, SM ;
Armitage, J .
MAYO CLINIC PROCEEDINGS, 2005, 80 (08) :1087-1097
[3]   LncRNA DBH-AS1 facilitates the tumorigenesis of hepatocellular carcinoma by targeting miR-138 via FAK/Src/ERK pathway [J].
Bao, Jie ;
Chen, Xiaoqi ;
Hou, Yuge ;
Kang, Gailing ;
Li, Qiaoli ;
Xu, Yun .
BIOMEDICINE & PHARMACOTHERAPY, 2018, 107 :824-833
[4]   Long Noncoding RNA and Cancer: A New Paradigm [J].
Bhan, Arunoday ;
Soleimani, Milad ;
Mandal, Subhrangsu S. .
CANCER RESEARCH, 2017, 77 (15) :3965-3981
[5]  
Chan WC, 1997, BLOOD, V89, P3909
[6]   Long non-coding RNA TTN-AS1 facilitates tumorigenesis of papillary thyroid cancer through modulating the miR-153-3p/ZNRF2 axis [J].
Cui, Zhenghui ;
Luo, Zhiyan ;
Lin, Zimei ;
Shi, Liuhong ;
Hong, Yurong ;
Yan, Caoxin .
JOURNAL OF GENE MEDICINE, 2019, 21 (05)
[7]   BUD13 Promotes a Type I Interferon Response by Countering Intron Retention in Irf7 [J].
Frankiw, Luke ;
Majumdar, Devdoot ;
Burns, Christian ;
Vlach, Logan ;
Moradian, Annie ;
Sweredoski, Michael J. ;
Baltimore, David .
MOLECULAR CELL, 2019, 73 (04) :803-+
[8]   Increased tumor cell proliferation in mantle cell lymphoma is associated with elevated insulin-like growth factor 2 mRNA-binding protein 3 expression [J].
Hartmann, Elena M. ;
Bea, Silvia ;
Navarro, Alba ;
Trapp, Vanessa ;
Campo, Elias ;
Ott, German ;
Rosenwald, Andreas .
MODERN PATHOLOGY, 2012, 25 (09) :1227-1235
[9]   RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3′UTR with miRNAs [J].
Hu, Ruixi ;
Zhu, Xiaodong ;
Chen, Chao ;
Xu, Ruijun ;
Li, Yifan ;
Xu, Wei .
CELL PROLIFERATION, 2018, 51 (06)
[10]   The landscape of long noncoding RNAs in the human transcriptome [J].
Iyer, Matthew K. ;
Niknafs, Yashar S. ;
Malik, Rohit ;
Singhal, Udit ;
Sahu, Anirban ;
Hosono, Yasuyuki ;
Barrette, Terrence R. ;
Prensner, John R. ;
Evans, Joseph R. ;
Zhao, Shuang ;
Poliakov, Anton ;
Cao, Xuhong ;
Dhanasekaran, Saravana M. ;
Wu, Yi-Mi ;
Robinson, Dan R. ;
Beer, David G. ;
Feng, Felix Y. ;
Iyer, Hariharan K. ;
Chinnaiyan, Arul M. .
NATURE GENETICS, 2015, 47 (03) :199-+
123