Determination of the amino acid sequence of a new phospholipase A2 (MIDCA1) isolated from Micrurus dumerilii carinicauda venom

被引：13
作者：
Dal Belo, CA
Toyama, MH [1 ]
Toyama, D
Marangoni, S
Moreno, FB
Cavada, BS
Fontana, MD
Hyslop, S
Carneiro, EM
Boschero, AC
机构：
[1] State Univ Campinas, UNICAMP, Inst Biol, Dept Physiol & Biophys, Campinas, SP, Brazil
[2] State Univ Campinas, UNICAMP, Sch Med Sci, Dept Pharmacol, Campinas, SP, Brazil
[3] State Univ Campinas, UNICAMP, Inst Biol, Dept Biochem, Campinas, SP, Brazil
[4] Fed Univ Ceara, Dept Biochem & Mol Biol, Fortaleza, Ceara, Brazil
来源：
PROTEIN JOURNAL | 2005年 / 24卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
amino acid sequence; Micrurus; neurotoxic and myotoxic; phospholipase A2;
D O I：
10.1007/s10930-005-7838-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new Phospholipase A(2) (PLA(2)) from Micrurus dumerilii carinicauda venom was isolated and its primary structure determined. This new PLA(2) showed a low enzymatic activity when compared with other PLA(2)s and it is moderately basic with an isoelectric point of 8.0. Its amino acid sequence showed the presence of 120 amino acid residues and its sequence was: NLIQFLNMIQCTTPGREPLVAFANYGCYCGRGGSGTPVDELDRCCQVHDNCYDTAKKVFGCSPYFTMYSYDCSEGKLTCKDNNTKCKAAVCNCDRTAALCFAKAPYNDKNYKIDLTKRCQ. The structural model of MIDCA1, when compared with other strong neurotoxic PLA(2)s, such as Naja naja, showed significant differences in the beta-wing and neurotoxic sites, despite the high level of amino acid sequence similarity. These observations indicate a dissociation between the biological and catalytic activity of this new PLA(2), supporting the view that other regions of the protein are involved in the biological effects.
引用
收藏
页码：147 / 153
页数：7
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