Characterization and plant expression of a glyphosate-tolerant enolpyruvylshikimate phosphate synthase

被引：10

作者：

Berg, Brian J. Vande ^{[1
]}

Hammer, Philip E. ^{[1
]}

Chun, Betty L. ^{[1
]}

Schouten, Laura C. ^{[1
]}

Carr, Brian

Guo, Rong ^{[1
]}

Peters, Cheryl ^{[1
]}

Hinson, Todd K. ^{[1
]}

Beilinson, Vadim ^{[1
]}

Shekita, Amy ^{[1
]}

Deter, Rebekah ^{[1
]}

Chen, Zhixian ^{[1
]}

Samoylov, Vladimir

Bryant, Charles T., Jr. ^{[1
]}

Stauffer, Maria E. ^{[1
]}

Eberle, Timothy ^{[1
]}

Moellenbeck, Dan J. ^{[2
]}

Carozzi, Nadine B. ^{[1
]}

Koziel, Mike G. ^{[1
]}

Duck, Nicholas B. ^{[1
]}

机构：

[1] Athenix Corp, Res Triangle Pk, NC 27709 USA

[2] DM Crop Res Grp Inc, Granger, IA 50109 USA

来源：

PEST MANAGEMENT SCIENCE | 2008年 / 64卷 / 04期

关键词：

glyphosate resistance; EPSP synthase; bacteria; Escherichia coli;

D O I：

10.1002/ps.1507

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

BACKGROUND: Glyphosate tolerance is a dominant trait in modern biotech crops. RESULTS: A gene encoding a glyphosate-tolerant EPSP synthase (aroA(1398)) from bacterial strain ATX1398 was cloned and characterized. The protein is initiated at a GTG translational. start codon to produce a protein that provides robust glyphosate resistance in Escherichia coli (Mig) Cast & Chalm. The aroA(1398) protein was expressed and purified from E. coli, and key kinetic values were determined (K-i = 161 mu m; K-m (PEP) = 11.3 mu M; k(cat) = 28.3 s(-1)). The full-length enzyme is 800-fold more resistant to glyphosate than the maize EPSP synthase while retaining high affinity for the substrate phosphoenol pyruvate. To evaluate further the potential of aroA(1398), transgenic maize events expressing the aroA(1398) protein were generated. To plants were screened for tolerance to glyphosate sprays at 1.3 x commercial spray rates, and T, plants were selected that completely resisted glyphosate sprays at 1 x, 2 x and 4 x recommended spray rates in field trials. CONCLUSION: These data suggest that aroA(1398) is a suitable candidate for conferring glyphosate tolerance in transgenic crop plants. (c) 2008 Society of Chemical Industry.

引用

页码：340 / 345

页数：6

共 14 条

[1]

Barry G. F., 1992, Patent No. [WO 9204449, 9204449]

[2] KINETICS OF 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE INHIBITION BY GLYPHOSATE [J].