Concentrating Genomic Length DNA in a Microfabricated Array

被引：29

作者：

Chen, Yu ^{[1
,2
]}

Abrams, Ezra S. ^{[4
]}

Boles, T. Christian ^{[4
]}

Pedersen, Jonas N. ^{[5
]}

Flyvbjerg, Henrik ^{[5
]}

Austin, Robert H. ^{[1
,3
]}

Sturm, James C. ^{[1
,2
]}

机构：

[1] Princeton Inst Sci & Technol Mat PRISM, Princeton, NJ 08540 USA

[2] Princeton Univ, Dept Elect Engn, Princeton, NJ 08544 USA

[3] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

[4] Sage Sci Inc, Beverly, MA 01915 USA

[5] Tech Univ Denmark, Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

来源：

PHYSICAL REVIEW LETTERS | 2015年 / 114卷 / 19期

基金：

美国国家卫生研究院;

关键词：

DETERMINISTIC LATERAL DISPLACEMENT; MOLECULES; DYNAMICS; CELLS; FLOW; ELECTROPHORESIS; FRACTIONATION; HYDRODYNAMICS; TRANSITION; COMPACTION;

D O I：

10.1103/PhysRevLett.114.198303

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We demonstrate that a microfabricated bump array can concentrate genomic-length DNA molecules efficiently at continuous, high flow velocities, up to 40 mu m/s, if the single-molecule DNA globule has a sufficiently large shear modulus. Increase in the shear modulus is accomplished by compacting the DNA molecules to minimal coil size using polyethylene glycol (PEG) derived depletion forces. We map out the sweet spot, where concentration occurs, as a function of PEG concentration and flow speed using a combination of theoretical analysis and experiment. Purification of DNA from enzymatic reactions for next-generation DNA-sequencing libraries will be an important application of this development.

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