High-Throughput in vitro Gel-Based Plate Assay to Screen for Calcium Oxalate Stone Inhibitors

被引:2
作者
Chmiel, John A. [1 ,2 ]
Stuivenberg, Gerrit A. [1 ,2 ]
Alathel, Abdulaziz [3 ,4 ,5 ]
Gorla, Jaswanth [2 ]
Grohe, Bernd [2 ]
Razvi, Hassan [6 ]
Burton, Jeremy P. [1 ,2 ,6 ]
Bjazevic, Jennifer [6 ]
机构
[1] Western Univ, Dept Microbiol & Immunol, London, ON, Canada
[2] Lawson Res Hlth Res Inst, London, ON, Canada
[3] Minist Natl Guard Hlth Affairs, Div Urol, Dept Surg, Riyadh, Saudi Arabia
[4] King Abdullah Int Med Res Ctr, Riyadh, Saudi Arabia
[5] King Saud Bin Abdulaziz Univ Hlth Sci, Riyadh, Saudi Arabia
[6] Western Univ, Dept Surg, Div Urol, London, ON, Canada
关键词
Kidney stones; Calcium oxalate; Crystal inhibitor; Antibiotic; Uropathogenic bacteria; KIDNEY-STONES; CRYSTALLIZATION; PATHOPHYSIOLOGY; OSTEOPONTIN;
D O I
10.1159/000519842
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Objective: Kidney stones are a common medical condition that is increasing in prevalence worldwide. Approximately, similar to 80% of urinary calculi are composed of calcium oxalate (CaOx). There is a growing interest toward identifying therapeutic compounds that can inhibit the formation of CaOx crystals. However, some chemicals (e.g., antibiotics and bacterial metabolites) may directly promote crystallization. Current knowledge is limited regarding crystal promoters and inhibitors. Thus, we have developed an in vitro gel-based diffusion model to screen for substances that directly influence CaOx crystal formation. Materials and Methods: We used double diffusion of sodium oxalate and calcium chloride-loaded paper disks along an agar medium to facilitate the controlled formation of monohydrate and dihydrate CaOx crystals. A third disk was used for the perpendicular diffusion of a test substance to assess its influence on CaOx crystal formation. Results: We confirmed that citrates and magnesium are effective inhibitors of CaOx crystals. We also demonstrated that 2 strains of uropathogenic Escherichia coli are able to promote crystal formation. While the other tested uropathogens and most antibiotics did not change crystal formation, ampicillin was able to reduce crystallization. Conclusion: We have developed an inexpensive and high-throughput model to evaluate substances that influence CaOx crystallization.
引用
收藏
页码:616 / 622
页数:7
相关论文
共 30 条
[1]   Oxalate-Degrading Bacillus subtilis Mitigates Urolithiasis in a Drosophila melanogaster Model [J].
Al, Kait F. ;
Daisley, Brendan A. ;
Chanyi, Ryan M. ;
Bjazevic, Jennifer ;
Razvi, Hassan ;
Reid, Gregor ;
Burton, Jeremy P. .
MSPHERE, 2020, 5 (05)
[2]   The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits [J].
Barr-Beare, Evan ;
Saxena, Vijay ;
Hilt, Evann E. ;
Thomas-White, Krystal ;
Schober, Megan ;
Li, Birong ;
Becknell, Brian ;
Hains, David S. ;
Wolfe, Alan J. ;
Schwaderer, Andrew L. .
PLOS ONE, 2015, 10 (10)
[3]   A guided tour into subcellular colocalization analysis in light microscopy [J].
Bolte, S. ;
Cordelieres, F. P. .
JOURNAL OF MICROSCOPY, 2006, 224 (213-232) :213-232
[4]   On the catalysis of calcium oxalate dihydrate formation by osteopontin peptides [J].
Chan, Brian P. H. ;
Vincent, Krista ;
Lajoie, Gilles A. ;
Goldberg, Harvey A. ;
Grohe, Bernd ;
Hunter, Graeme K. .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2012, 96 :22-28
[5]  
CODY AM, 1982, SCAN ELECTRON MICROS, P185
[6]   Drug-Induced Kidney Stones and Crystalline Nephropathy: Pathophysiology, Prevention and Treatment [J].
Daudon, Michel ;
Frochot, Vincent ;
Bazin, Dominique ;
Jungers, Paul .
DRUGS, 2018, 78 (02) :163-201
[7]   Metal chelates of ampicillin versus amoxicillin: synthesis, structural investigation, and biological studies [J].
El-Gamel, Nadia E. A. .
JOURNAL OF COORDINATION CHEMISTRY, 2010, 63 (03) :534-543
[8]   Complex wavelets for extended depth-of-field: A new method for the fusion of multichannel microscopy images [J].
Forster, B ;
Van de Ville, D ;
Berent, J ;
Sage, D ;
Unser, M .
MICROSCOPY RESEARCH AND TECHNIQUE, 2004, 65 (1-2) :33-42
[9]   Crystallization kinetics of calcium oxalate hydrates studied by scanning confocal interference microscopy [J].
Grohe, Bernd ;
Rogers, Kem A. ;
Goldberg, Harvey A. ;
Hunter, Graeme K. .
JOURNAL OF CRYSTAL GROWTH, 2006, 295 (02) :148-157
[10]   A NEW APPROACH TO STUDYING INHIBITORS OF CALCIUM-OXALATE CRYSTAL-GROWTH [J].
HENNEQUIN, C ;
LALANNE, V ;
DAUDON, M ;
LACOUR, B ;
DRUEKE, T .
UROLOGICAL RESEARCH, 1993, 21 (02) :101-108