Ligustilide attenuates vascular inflammation and activates Nrf2/HO-1 induction and, NO synthesis in HUVECs

被引：83

作者：

Choi, Eun Sik ^{[1
,2
,3
]}

Yoon, Jung Joo ^{[1
,2
,3
]}

Han, Byung Hyuk ^{[1
,2
,3
]}

Jeong, Da Hye ^{[1
,2
,3
]}

Lee, Yun Jung ^{[1
,2
,3
]}

Kang, Dae Gill ^{[1
,2
,3
]}

Lee, Ho Sub ^{[1
,2
,3
]}

机构：

[1] Wonkwang Univ, Coll Oriental Med, 460 Iksandae Ro, Jeonbuk 54538, South Korea

[2] Wonkwang Univ, Profess Grad Sch Oriental Med, 460 Iksandae Ro, Jeonbuk 54538, South Korea

[3] Wonkwang Univ, Hanbang Cardiorenal Syndrome Res Ctr, 460 Iksandae Ro, Jeonbuk 54538, South Korea

来源：

PHYTOMEDICINE | 2018年 / 38卷

基金：

新加坡国家研究基金会;

关键词：

Ligustilide; HUVECs; TNF-alpha; Vascular inflammation; Atherosclerosis; Adhesion molecules; NF-kappa B; HO-1; Nrf2; NO synthesis; NF-KAPPA-B; CNIDIUM-OFFICINALE MAKINO; SI-WU-TANG; NITRIC-OXIDE; ENDOTHELIAL-CELLS; LEUKOCYTE ADHESION; ANGELICA-SINENSIS; RAT LIVERS; EXPRESSION; MEDICINE;

D O I：

10.1016/j.phymed.2017.09.022

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Ligustilide is a bioactive phthalide derivative isolated from Cnidii Rhizoma (Cnidium officinale, rhizome) and Angelicae Gigantis Radix (Angelica gigas Nakai, root) which are both medicinal herbs used to treat circulatory disorders. Vascular endothelium is a central spot in developing cardiovascular diseases and chronic vascular inflammation might result in atherosclerosis development. Purpose: We previously found out that a traditional herbal formula, Samul-Tang (Si-Wu-Tang, containing Cnidii Rhizoma and Angelicae Gigantis Radix), attenuated vascular inflammation in human umbilical vein endothelial cells (HUVECs). However, which compound was responsible for vascular protective action remained unclear. Here, we investigated vascular protective potential of an isolated single compound, (Z)-ligustilide. Methods: MTT assay, western blotting, immunofluorescence, electrophoretic mobility shift assay was performed. BCECF-AM, CM-H(2)DCFDA, DAF-FM diacetate were used as a fluorescent indicator. Results: Ligustilide suppressed HL-60 monocyte adhesion and CAMs (ICAM-1, VCAM-1, E-selectin) expression in HUVECs. Ligustilide significantly inhibited TNF-alpha-increased production of ROS and activated NF-kappa B signaling pathway. Also, ligustilide treated HUVECs exhibited significant HO-1 induction via Nrf2 nuclear translocation and endothelial NO synthesis. Conclusion: Present study demonstrates that ligustilde attenuates vascular inflammation and activate defense system of endothelial cell. Ligustilide is a bioactive compound which might prevent cardiovascular complications such as thrombosis or atherosclerosis.

引用

页码：12 / 23

页数：12

共 44 条

[1] Phenotypic heterogeneity of the endothelium I. Structure, function, and mechanisms [J].