Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens

被引：69
作者：
Kulkarni, R. R. [1 ]
Parreira, V. R. [1 ]
Sharif, S. [1 ]
Prescott, J. F. [1 ]
机构：
[1] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada
来源：
VACCINE | 2008年 / 26卷 / 33期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Clostrdium perfringens; vaccine; necrotic enteritis; chickens;
D O I：
10.1016/j.vaccine.2008.05.079
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding three C perfringens proteins: fructose-biphosphate-aldolase (FBA), pyruvate:ferredoxinoxidoreductase (PFOR) and hypothetical protein (HP), were cloned into an avirulent Salmonella enterica sv. typhimurium vaccine vector. Broiler chickens immunized orally with recombinant Salmonella expressing FBA or HP proteins were significantly protected against NE challenge. Immunized birds developed serum and mucosal antibodies to both clostridial and Salmonella antigens. This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4194 / 4203
页数：10
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