Inhibition of hepatitis B virus replication by in vitro synthesized RNA

被引：0

作者：

Yang, YJ ^{[1
]}

Hoe, YS ^{[1
]}

Kim, JK ^{[1
]}

Kim, SY ^{[1
]}

Ahn, JK ^{[1
]}

机构：

[1] Chungnam Natl Univ, Dept Microbiol, Taejon 305764, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2005年 / 26卷 / 09期

关键词：

hepatitis B virus; HBx RNA; siRNA; HBV replication; new therapeutic agent;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Human hepatitis B virus (HBV) is a pathogen related to the development of liver diseases including chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). However, the efficient methods to suppress HBV replication have not been developed yet. Therefore, we have used RNA interference (RNAi) as a potential tool for the suppression of HBV replication. Here, we designed a 21 nt small intefering dsRNA (siRNA) against hepatitis B virus X (HBx) RNA with 3'overhanging ends derived from T7 promoter. It has been reported that HBV X protein plays an important role in HBV gene expression and viral replication. The Suppression of HBx gene expression by the 21 nt siRNA was investigated by Northern blot analysis and chloramphenicol acetyl transferase (CAT) assay. The level of HBx mRNA was decreased by siRNA in a dose-dependent manner. We also found that the 21 nt siRNA inhibited the HBV replication in hepatocellular carcinoma cell.

引用

页码：1385 / 1389

页数：5

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