The physical and cytological properties of white MTA mixed with Na2HPO4 as an accelerant

被引:52
作者
Ding, Shinn Jyn [1 ]
Kao, Chia Tze [1 ]
Shie, Ming You [1 ]
Hung, Chi, Jr. [2 ]
Huang, Tsui Hsein [1 ,2 ]
机构
[1] Chung Shan Med Univ & Hosp, Inst Oral Mat Sci, Taichung, Taiwan
[2] Chung Shan Med Univ & Hosp, Dept Dent, Taichung, Taiwan
关键词
accelerant; biological properties; mineral trioxide aggregate; physical properties;
D O I
10.1016/j.joen.2008.02.041
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The purpose of the present study was to evaluate the physicochemical and cytologic properties of mineral trioxide aggregate (MTA) mixed with distilled water and Na2HPO4 buffer solution. The MTA setting time and pH value were evaluated. An MTA micrograph on scanning electron microscopy (SEM) was observed. Mouse fibroblasts (an L929 cell line) were used to test the toxicity of MTA after the first and seventh day of treatment by mitochondiral colorimetric assay. The results show the Na2HPO4 buffer group reduced the MTA setting time, and the pH value as in the distilled water group is similar with the Na2HPO4 buffer group. The mixed MTA's XRD produced similar peaks of the distilled water and Na2HPO4 buffer solution groups. The L929 cell survival rate of distilled water and Na2HPO4 buffer solution groups did not exhibit any significant difference (p < 0.05). There are differences in SEM observations both of the MTA surface and of the cells in culture on the surface of the MTA with Na2HPO4 versus distilled water. The results suggest that 15% Na2HPO4 buffer can be successfully used as an accelerator of MTA.
引用
收藏
页码:748 / 751
页数:4
相关论文
共 18 条
[11]   Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts [J].
Keiser, K ;
Johnson, CC ;
Tipton, DA .
JOURNAL OF ENDODONTICS, 2000, 26 (05) :288-291
[12]   Effects of physiological environments on the hydration behavior of mineral trioxide aggregate [J].
Lee, YL ;
Lee, BS ;
Lin, FH ;
Lin, AY ;
Lan, WH ;
Lin, CP .
BIOMATERIALS, 2004, 25 (05) :787-793
[13]   Osteoblast biocompatibility of mineral trioxide aggregate [J].
Mitchell, PJC ;
Ford, TRP ;
Torabinejad, M ;
McDonald, F .
BIOMATERIALS, 1999, 20 (02) :167-173
[14]   Cytotoxicity of endodontic materials [J].
Osorio, RM ;
Hefti, A ;
Vertucci, FJ ;
Shawley, AL .
JOURNAL OF ENDODONTICS, 1998, 24 (02) :91-96
[15]   Cell and tissue reactions to mineral trioxide aggregate and Portland cement [J].
Saidon, J ;
He, JN ;
Zhu, Q ;
Safavi, K ;
Spångberg, LSW .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 2003, 95 (04) :483-489
[16]   Physico-chemical properties of MTA and a novel experimental cement [J].
Santos, AD ;
Moraes, JCS ;
Araújo, EB ;
Yukimitu, K ;
Valério, WV .
INTERNATIONAL ENDODONTIC JOURNAL, 2005, 38 (07) :443-447
[17]   Evaluation of setting properties and retention characteristics of mineral trioxide aggregate when used as a furcation perforation repair material [J].
Sluyk, SR ;
Moon, PC ;
Hartwell, GR .
JOURNAL OF ENDODONTICS, 1998, 24 (11) :768-771
[18]   Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs [J].
Torabinejad, M ;
Ford, TRP ;
Abedi, HR ;
Kariyawasam, SP ;
Tang, HM .
JOURNAL OF ENDODONTICS, 1998, 24 (07) :468-471