The Effect of Green Tea Waste on Growth and Health of Grass Carp (Ctenopharyngodon idellus)

To investigate the effects of green tea waste on growth and health of grass carp (Ctenopharyngodon idellus), Green tea waste (GTW) and green tea (GT) were added in the basal diet (BD) of fish by replacing wheat at an inclusion level of 5% to form three diets. 60 grass carp (43.8 +/- 2.8 g) were divided into three groups (each group having two replicates) and fed for 66 days. After which growth and health of fish were determined. The results showed that final weight of fish and feed efficiency ratio in GTW group were between BD and GT groups and they were not different compared with BD or with GT groups. Expression of muscle myf5 and myf6 in GTW were between BD and GT group, being significantly lower than BD group. Serum TP, ALB, GLO, HDL, LDL and T-AOC in GTW group were significantly higher than BD group. Expression of hsp 70 and ubiquitin in hepatopancreas of fish in GTW group were significantly higher than BD group. The results suggest that GTW supplementation has positive impact on health of fish without affecting the growth of fish.