TRANSGENIC TOBACCO WITH RICE FAE GENE EXHIBITS HIGHER WATER USE EFFICIENCY

被引：0

作者：

Bhatti, Khizar Hayat ^{[1
,2
]}

Shah, Amin ^{[3
]}

Mehmood, Qaiser ^{[4
]}

Khan, Sardar ^{[5
]}

He, Wujia ^{[2
]}

He Chaozu ^{[2
]}

机构：

[1] Univ Gujrat, Dept Bot, Inst Chem & Biol Sci, Gujrat 50700, Pakistan

[2] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

[3] Univ Sargodha, Dept Bot, Sargodha 40100, Pakistan

[4] COMSATS II, Dept Environm Sci, Abbottabad 22010, Pakistan

[5] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

来源：

PAKISTAN JOURNAL OF BOTANY | 2011年 / 43卷 / 05期

关键词：

CHAIN FATTY-ACID; MULTIPLE SEQUENCE ALIGNMENT; LEAF WAX ACCUMULATION; CUTICULAR WAX; FUNCTIONAL-CHARACTERIZATION; EPICUTICULAR WAX; DROUGHT; STRESS; BIOSYNTHESIS; EXPRESSION;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The rice FAE encodes protein for fatty acids elongation to form very long chain fatty acids (VLCFAs), which are the intermediates for wax biosynthesis. Agrobacterium-mediated transgenic tobacco plants bear rice fatty acid elongation gene (OsFAE), which has been incorporated into their genome. Amino acids multiple sequences alignment analysis reveals that rice FAE protein has sequence similarity with other fatty acids elongation and wax related proteins, especially corn FAE. Phylogenetic tree, a bioinformatics tool shows that OsFAE has a close evolutionary origin with that of maize FAE. Sense sequence of rice FAE gene incorporation to transgenic rice has consequently resulted into relatively more cuticular wax on leaf surface. Scanning electron microscopy (SEM) illustrates that transgenic tobacco leaves have phenotypically higher cuticular waxes than of control. Our findings also suggest that the transgenic tobacco exhibits more water use efficiency (WUE) at both the 90% and 35% field capacity (FC) levels under non-stress and stressful conditions, respectively.

引用

页码：2527 / 2533

页数：7