Proteomic approach to the diagnosis of acute coronary syndrome: Preliminary results

Background: Cardiac multimarker strategy is recommended by the IFCC, ESC and the ACC for an early risk stratification in non-ST-segment elevation (NSTE) ECG patients with chest pain. A new approach, based on protein biochip array technology, performs simultaneously: cTnI, CK-MB, myoglobin, CAIII, GFBB and FABP using a single chip. Results: Troponin, CK-MB and FABP methods provide reproducible within-run and between-day results (total %CVs from 5.9% to 9.7%), and myoglobin and CAM methods showed the total %CVs from 16.4% to 25.8%. Our preliminary clinical data suggests that FABP had a better diagnostic performance (sensibility = 100%) than myoglobin (sensibility = 75%) to detect AMI in the first hours after the onset of the chest pain and myoglobin/CAIII ratio (specificity = 92.9%) improved the myoglobin specificity. Conclusions: Cardiac markers have different diagnostic roles and, in this contest, biochip technology could be an interesting approach supporting clinical expectations. (c) 2005 Elsevier B.V. All rights reserved.