Effect of photoautotrophic and heteroautotrophic conditions on growth and lipid production in Chlorella vulgaris cultured in industrial wastewater with the yeast Rhodotorula glutinis

被引:18
作者
Zhang, Kun [1 ,2 ,3 ]
Zheng, Jiahui [1 ]
Xue, Daling [1 ]
Ren, Difeng [1 ]
Lu, Jun [2 ]
机构
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, Beijing 100083, Peoples R China
[2] Beijing Engn Res Ctr Prot & Funct Peptides, China Natl Res Inst Food & Fermentat Ind, Beijing 100015, Peoples R China
[3] Cent Univ Finance & Econ, Beijing 100081, Peoples R China
基金
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词
Chlorella vulgaris; Photoautotroph; Heterotroph; Co-culture; Lipid production; FATTY-ACID-COMPOSITION; STRAINS; ACCUMULATION; MICROALGAE; PURE;
D O I
10.1007/s10811-017-1168-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The photoautotrophic co-culture of Chlorella vulgaris and the yeast Rhodotorula glutinis (MP culture) from industrial wastewater was investigated. Cell numbers, biomass, lipid production, and fatty acid content were measured. Owing to co-culture interaction with yeast and microalgae, the MP culture resulted in the highest number of cells (27.73 x 10(5) cells mL(-1)) and biomass (0.808 g L-1). Lipid production in the MP culture (117.73 mg L-1) was fourfold higher than that in the photoautotrophic pure culture (23.1 mg L-1). The content of palmitic acid (C16:0) was 24.65%, whereas that of oleic acid (C18:1) was 56.34% in the MP culture, which was higher than in other cultures. The results of this study indicate that MP cultures can be used to effectively support the growth of microorganisms and as an approach for biodiesel production.
引用
收藏
页码:2783 / 2788
页数:6
相关论文
共 21 条
  • [1] Borowitzka M.A., 2013, Algae for Biofuels and Energy, Developments in Applied Phycology, P1, DOI [DOI 10.1007/978-94-007-5479-9, 10.1007/978-94-007-5479-9_1, DOI 10.1007/978-94-007-5479-9_1]
  • [2] Commercial production of microalgae: ponds, tanks, tubes and fermenters
    Borowitzka, MA
    [J]. JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) : 313 - 321
  • [3] Comparisons of growth and biochemical composition between mixed culture of alga and yeast and monocultures
    Cai, ShiQing
    Hu, ChaoQun
    Du, ShaoBo
    [J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2007, 104 (05) : 391 - 397
  • [4] Co-culture of an oleaginous yeast Rhodotorula glutinis and a microalga Chlorella vulgaris for biomass and lipid production using pure and crude glycerol as a sole carbon source
    Cheirsilp, Benjamas
    Kitcha, Suleeporn
    Torpee, Salwa
    [J]. ANNALS OF MICROBIOLOGY, 2012, 62 (03) : 987 - 993
  • [5] Lipid Production by Culturing Oleaginous Yeast and Algae with Food Waste and Municipal Wastewater in an Integrated Process
    Chi, Zhanyou
    Zheng, Yubin
    Jiang, Anping
    Chen, Shulin
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 165 (02) : 442 - 453
  • [6] FLUOROMETRIC-DETERMINATION OF THE NEUTRAL LIPID-CONTENT OF MICROALGAL CELLS USING NILE RED
    COOKSEY, KE
    GUCKERT, JB
    WILLIAMS, SA
    CALLIS, PR
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 1987, 6 (06) : 333 - 345
  • [7] FOLCH J, 1957, J BIOL CHEM, V226, P497
  • [8] Fulton L, 2004, BIOMASS AGR SUSTAINA, P105
  • [9] JHAM GN, 1982, J AM OIL CHEM SOC, V59, P132, DOI 10.1007/BF02662261
  • [10] Lipid and γ-linolenic acid accumulation in strains of zygomycetes growing on glucose
    Kavadia, A
    Komaitis, M
    Chevalot, I
    Blanchard, F
    Marc, I
    Aggelis, G
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2001, 78 (04) : 341 - 346