Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus mutans

被引：0
|
作者：
Zhou, Yan-Feng [1 ]
Li, Lan-Fen [1 ]
Yang, Cheng [1 ,2 ]
Liang, Yu-He [1 ]
Su, Xiao-Dong [1 ,3 ]
机构：
[1] Peking Univ, Coll Life Sci, Natl Lab Protein Engn & Plant Genet Engn, Beijing 100871, Peoples R China
[2] Rigaku MSC Inc, The Woodlands, TX 77381 USA
[3] Peking Univ, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷
关键词：
D O I：
10.1107/S1744309107065645
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
SMU.573 from Streptococcus mutans is a structurally and functionally uncharacterized protein that was selected for structural biology studies. Native and SeMet-labelled proteins were expressed with an N-His tag in Escherichia coli BL21 (DE3) and purified by Ni2+-chelating and size-exclusion chromatography. Crystals of the SeMet-labelled protein were obtained by the hanging-drop vapour-diffusion method and a 2.5 angstrom resolution diffraction data set was collected using an in-house chromium radiation source. The crystals belong to space group I4, with unit-cell parameters a = b = 96.53, c = 56.26 angstrom, alpha = beta = gamma = 90 degrees.
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页码：47 / 49
页数：3
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