Influence of type of culture medium on characterization of Mycobacterium avium subsp paratuberculosis subtypes

被引:20
作者
Cernicchiaro, N. [1 ]
Wells, S. J. [1 ]
Janagama, H. [1 ]
Sreevatsan, S. [1 ]
机构
[1] Univ Minnesota, Vet Populat Med Dept, Andrew Boss Lab Meat Hyg 136G, Coll Vet Med, St Paul, MN 55108 USA
关键词
D O I
10.1128/JCM.01769-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the effects of culture and/or enrichment methods on the selection of Mycobacterium avium subsp. paratuberculosis subtypes. M. avium subsp. paratuberculosis isolates from bovine fecal samples processed using a centrifugation protocol were investigated in both liquid (MGIT ParaTb tubes) and solid (Herrold's egg yolk medium) culture media. For this evaluation, M. avium subsp. paratuberculosis subtyping was based on the sequence variation in two of the most discriminatory short sequence repeat loci, i.e., mononucleotide G and trinucleotide GGT, in isolates from liquid and solid cultures. This study identified the existence of one major predominating fingerprint (>13G-5GGT) in bovine fecal samples, regardless of the type of medium used for isolation. Matched-pair analysis of subtypes showed that 69% of samples presented unique subtypes in the two culture media used, while 31% shared the same G-GGT allele. Furthermore, the liquid culture method appeared to select for a more genotypically diverse subtype population than the solid culture method. The variety of subtypes observed between liquid and solid cultures obtained from the same fecal samples suggests that the culture method could provide a "microbiological" bias and lead to a discrepancy in the growth of M. avium subsp. paratuberculosis strains. In conclusion, this study identified that these two types of culture media determined differential growth of M. avium subsp. paratuberculosis strains and that this should be considered in evaluating detection capabilities of diagnostic tests or interpreting data from molecular epidemiological studies performed using conventional solid fecal culture or automated liquid medium culture methods.
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页码:145 / 149
页数:5
相关论文
共 23 条
[1]   Multilocus short sequence repeat sequencing approach for differentiating among Mycobacterium avium subsp paratuberculosis strains [J].
Amonsin, A ;
Li, LL ;
Zhang, O ;
Bannantine, JP ;
Motiwala, AS ;
Sreevatsan, S ;
Kapur, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1694-1702
[2]   Isolation of Mycobacterium avium subsp paratuberculosis from free-ranging birds and mammals on livestock premises [J].
Corn, JL ;
Manning, EJB ;
Sreevatsan, S ;
Fischer, JR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :6963-6967
[3]   Detection of Mycobacterium avium subsp paratuberculosis in tissue samples by single, fluorescent and nested PCR based on the IS900 gene [J].
Englund, S ;
Bölske, G ;
Ballagi-Pordány, A ;
Johansson, KE .
VETERINARY MICROBIOLOGY, 2001, 81 (03) :257-271
[4]   Mycobacterium avium subsp paratuberculosis strains isolated from Crohn's disease patients and animal species exhibit similar polymorphic locus patterns [J].
Ghadiali, AH ;
Strother, M ;
Naser, SA ;
Manning, EJB ;
Sreevatsan, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :5345-5348
[5]   Short-sequence-repeat analysis of Mycobacterium avium subsp paratuberculosis and Mycobacterium avium subsp avium isolates collected from animals throughout the united states reveals both stability of loci and extensive diversity [J].
Harris, N. Beth ;
Payeur, Janet B. ;
Kapur, Vivek ;
Sreevatsan, Srinand .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2970-2973
[6]   Methods of mycobacterial DNA isolation from different biological material: a review [J].
Hosek, J. ;
Svastova, P. ;
Moravkova, M. ;
Pavlik, I. ;
Bartos, M. .
VETERINARNI MEDICINA, 2006, 51 (05) :180-192
[7]   NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY [J].
HUNTER, PR ;
GASTON, MA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) :2465-2466
[8]   Amplified fragment length polymorphism to detect clonal diversity and distribution of Mycobacterium avium subspecies paratuberculosis in selected Minnesota dairy cattle [J].
Kiehnbaum, LA ;
Amonsin, A ;
Wells, SJ ;
Kapur, V .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2005, 17 (04) :311-315
[9]  
KRADER CA, 1996, APPL ENVIRON MICROB, V62, P1102
[10]   MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].
Kumar, S ;
Tamura, K ;
Nei, M .
BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163