Enhancement of γ-Amminobutyric Acid Production by Lactobacillus sakei B2-16 Expressing Glutamate Decarboxylase from Lactobacillus plantarum ATCC 14917

被引：38

作者：

Kook, Moo-Chang ^{[2
,3
]}

Seo, Myung-Ji ^{[4
]}

Cheigh, Chan-Ick ^{[5
]}

Lee, Sang-Jae ^{[6
]}

Pyun, Yu-Ryang ^{[3
,6
]}

Park, Hoon ^{[1
]}

机构：

[1] Sunmoon Univ, Dept Food Sci, Asan 336708, South Korea

[2] Anyang Univ, Dept Marine Biotechnol, Inchon 417833, South Korea

[3] Yonsei Univ, Biovan Co Ltd, R&D Div, Seoul 120749, South Korea

[4] Brown Univ, Dept Chem, Providence, RI 02912 USA

[5] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 120750, South Korea

[6] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

来源：

JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | 2010年 / 53卷 / 06期

关键词：

gamma-amminobutyric acid; glutamate decarboxylase; Lactobacillus plantarum; Lactobacillus sakei; LACTOCOCCUS-LACTIS; CLONING; GENE; GABA; VECTORS; BREVIS;

D O I：

10.3839/jksabc.2010.123

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To enhance gamma-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

引用

页码：816 / 820

页数：5

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