Determination of pyrrolizidine alkaloids in tea and honey with automated SPE clean-up and ultra-performance liquid chromatography/tandem mass spectrometry

For routine analytical purpose a method based on a combination of automated solid-phase extraction (SPE) clean-up and detection by Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry (UPLC-MS/MS) was developed for the determination of pyrrolizidine alkaloids (PA) in various food commodities. In this survey, honey, tea and herbal infusion samples from local retailers collected in 2012-2015 were obtained and analysed for their PA content. PA concentrations were found in 30% of the honey samples and in 42% of the tea and herbal infusion samples with levels up to 595 mu g/kg. The survey included 17 individual PA, and their sum is also reported for each sample.