Comparative study of the human ficolins reveals unique features of Ficolin-3 (Hakata antigen)

被引:101
作者
Hummelshoj, Tina [1 ]
Fog, Lea Munthe [1 ]
Madsen, Hans O. [1 ]
Sim, Robert B. [2 ]
Garred, Peter [1 ]
机构
[1] Univ Copenhagen, Rigshosp, Dept Clin Immunol, Sect 7631, DK-2100 Copenhagen, Denmark
[2] Univ Oxford, MRC, Immunochem Unit, Dept Biochem, Oxford OX1 3QU, England
关键词
complement; ficolin; lectin; mannose-binding lectin; expression; innate immunity;
D O I
10.1016/j.molimm.2007.10.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ficolins and mannose-binding lectin (MBL) are collagen-like defence proteins that serve as recognition molecules in lectin complement pathway. Differential features that may indicate diverse functions of these proteins are poorly understood. In this study we compared important biological features of the ficolins and MBL. We investigated the tissue distribution of the FCN1-3 and the MBL2 genes encoding the ficolins and MBL by quantitative PCR. Recombinant proteins were produced and structural and biological characteristics were investigated and compared. Our main findings were that FCN3 mRNA was highly expressed in the liver and lung compared with the other genes revealing the lung as the tissue with the highest FCN3 expression pattern. Ficolin-3 revealed higher complement activating capacity compared with Ficolin-2, MBL and Ficolin-1 and was highly resistant to bacterial collagenase treatment, which is different from the other ficolins and MBL. We discovered several unique properties of Ficolin-3 showing that FCN3 is the most highly expressed gene in liver and lung among the lectin complement pathway initiators. Moreover, Ficolin-3 has a high complement activating potential and is the only collagenase proteolytic resistant molecule among the lectin complement pathway initiators. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1623 / 1632
页数:10
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