Isolation and Molecular Characterization of 1-Aminocyclopropane-1-carboxylic Acid Synthase Genes in Hevea brasiliensis

被引：14

作者：

Zhu, Jia-Hong ^{[1
]}

Xu, Jing ^{[2
]}

Chang, Wen-Jun ^{[1
]}

Zhang, Zhi-Li ^{[1
,2
]}

机构：

[1] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

[2] Hainan Acad Agr Sci, Haikou 571100, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 02期

基金：

中国国家自然科学基金;

关键词：

HIGH-QUALITY RNA; ETHYLENE BIOSYNTHESIS; ACC SYNTHASE; DIFFERENTIAL EXPRESSION; ARABIDOPSIS; RUBBER; FAMILY; PHOSPHORYLATION; NORMALIZATION; TURNOVER;

D O I：

10.3390/ijms16024136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene is an important factor that stimulates Hevea brasiliensis to produce natural rubber. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a rate-limiting enzyme in ethylene biosynthesis. However, knowledge of the ACS gene family of H. brasiliensis is limited. In this study, nine ACS-like genes were identified in H. brasiliensis. Sequence and phylogenetic analysis results confirmed that seven isozymes (HbACS1-7) of these nine ACS-like genes were similar to ACS isozymes with ACS activity in other plants. Expression analysis results showed that seven ACS genes were differentially expressed in roots, barks, flowers, and leaves of H. brasiliensis. However, no or low ACS gene expression was detected in the latex of H. brasiliensis. Moreover, seven genes were differentially up-regulated by ethylene treatment.These results provided relevant information to help determine the functions of the ACS gene in H. brasiliensis, particularly the functions in regulating ethylene stimulation of latex production.

引用

页码：4136 / 4149

页数：14

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