An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue

被引：22

作者：

Baydoun, Martha ^{[1
,2
,3
]}

Treizeibre, Anthony ^{[1
]}

Follet, Jerome ^{[1
,2
]}

Vanneste, Sadia Benamrouz ^{[3
,4
]}

Creusy, Colette ^{[5
]}

Dercourt, Lucie ^{[6
]}

Delaire, Baptiste ^{[5
]}

Mouray, Anthony ^{[7
]}

Viscogliosi, Eric ^{[3
]}

Certad, Gabriela ^{[3
,8
]}

Senez, Vincent ^{[1
,6
]}

机构：

[1] Univ Lille, CNRS, ISEN YNCREA, UMR 8520 IEMN, F-59000 Lille, France

[2] ISA YNCREA Hauts France, F-59000 Lille, France

[3] Univ Lille, CHU Lille, INSERM, CNRS,Inst Pasteur Lille,U1019,UMR 9107,CIIL,Ctr I, F-59019 Lille, France

[4] Inst Catholique Lille, Fac Gest Econ & Sci, Unite Rech Smart & Sustainable Cities, Lab Ecol & Biodiversite, F-59800 Lille, France

[5] Univ Catholique Lille, Grp Hop, Serv Anat & Cytol Pathol, F-59000 Lille, France

[6] Univ Tokyo, CNRS, UMI 2820, LIMMS, F-59000 Lille, France

[7] Inst Pasteur Lille Lille, Plateforme Expt & Hautes Technol Anim, F-59019 Lille, France

[8] Univ Catholique Lille, Fac Med & Maieut, GHICL, Delegat Rech Clin Eta Innovat, F-59800 Lille, France

来源：

MICROMACHINES | 2020年 / 11卷 / 02期

关键词：

organotypic tissue model; intestinal tract model; ex vivo microfluidic tissue culture; long term viability maintenance; ORGAN-CULTURE; TRANSPORT; CHIP; MODELS; CELLS; EPITHELIUM; MEMBRANES; DISEASE; GROWTH;

D O I：

10.3390/mi11020150

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.

引用

页数：16

共 50 条

[21] Ex vivo culture of human prostate tissue and drug development
Margaret M. Centenera
Ganesh V. Raj
Karen E. Knudsen
Wayne D. Tilley
Lisa M. Butler
Nature Reviews Urology, 2013, 10 : 483 - 487
[22] Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
Namba, Takashi
Haffner, Christiane
Huttner, Wieland B.
BIO-PROTOCOL, 2021, 11 (10):
[23] Ex vivo culture of intestinal crypt organoids as a model system for assessing cell death induction in intestinal epithelial cells and enteropathy
T Grabinger
L Luks
F Kostadinova
C Zimberlin
J P Medema
M Leist
T Brunner
Cell Death & Disease, 2014, 5 : e1228 - e1228
[24] Ex vivo culture of intestinal crypt organoids as a model system for assessing cell death induction in intestinal epithelial cells and enteropathy
Grabinger, T.
Luks, L.
Kostadinova, F.
Zimberlin, C.
Medema, J. P.
Leist, M.
Brunner, T.
CELL DEATH & DISEASE, 2014, 5 : e1228 - e1228
[25] Ex Vivo and in Vivo Study of Sucrosomial® Iron Intestinal Absorption and Bioavailability
Fabiano, Angela
Brilli, Elisa
Mattii, Letizia
Testai, Lara
Moscato, Stefania
Citi, Valentina
Tarantino, Germano
Zambito, Ylenia
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (09)
[26] A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography
de Hoyos-Vega, Jose M.
Gonzalez-Suarez, Alan M.
Garcia-Cordero, Jose L.
MICROSYSTEMS & NANOENGINEERING, 2020, 6 (01)
[27] A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography
Jose M. de Hoyos-Vega
Alan M. Gonzalez-Suarez
Jose L. Garcia-Cordero
Microsystems & Nanoengineering, 6
[28] Ex vivo culture of human colorectal tissue for the evaluation of candidate microbicides
Fletcher, Patricia S.
Elliott, Julie
Grivel, Jean-Charles
Margolis, Leonid
Anton, Peter
McGowan, Ian
Shattock, Robin J.
AIDS, 2006, 20 (09) : 1237 - 1245
[29] Dynamic Physiological Culture of Ex Vivo Human Tissue: A Systematic Review
Hughes, Daniel Ll
Hughes, Aron
Soonawalla, Zahir
Mukherjee, Somnath
O'Neill, Eric
CANCERS, 2021, 13 (12)
[30] Design of a sterile organ culture system for the ex vivo study of aortic heart valves
Warnock, JN
Konduri, S
He, ZM
Yoganathan, AP
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2005, 127 (05): : 857 - 861

← 1 2 3 4 5 →