An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue

被引：22

作者：

Baydoun, Martha ^{[1
,2
,3
]}

Treizeibre, Anthony ^{[1
]}

Follet, Jerome ^{[1
,2
]}

Vanneste, Sadia Benamrouz ^{[3
,4
]}

Creusy, Colette ^{[5
]}

Dercourt, Lucie ^{[6
]}

Delaire, Baptiste ^{[5
]}

Mouray, Anthony ^{[7
]}

Viscogliosi, Eric ^{[3
]}

Certad, Gabriela ^{[3
,8
]}

Senez, Vincent ^{[1
,6
]}

机构：

[1] Univ Lille, CNRS, ISEN YNCREA, UMR 8520 IEMN, F-59000 Lille, France

[2] ISA YNCREA Hauts France, F-59000 Lille, France

[3] Univ Lille, CHU Lille, INSERM, CNRS,Inst Pasteur Lille,U1019,UMR 9107,CIIL,Ctr I, F-59019 Lille, France

[4] Inst Catholique Lille, Fac Gest Econ & Sci, Unite Rech Smart & Sustainable Cities, Lab Ecol & Biodiversite, F-59800 Lille, France

[5] Univ Catholique Lille, Grp Hop, Serv Anat & Cytol Pathol, F-59000 Lille, France

[6] Univ Tokyo, CNRS, UMI 2820, LIMMS, F-59000 Lille, France

[7] Inst Pasteur Lille Lille, Plateforme Expt & Hautes Technol Anim, F-59019 Lille, France

[8] Univ Catholique Lille, Fac Med & Maieut, GHICL, Delegat Rech Clin Eta Innovat, F-59800 Lille, France

来源：

MICROMACHINES | 2020年 / 11卷 / 02期

关键词：

organotypic tissue model; intestinal tract model; ex vivo microfluidic tissue culture; long term viability maintenance; ORGAN-CULTURE; TRANSPORT; CHIP; MODELS; CELLS; EPITHELIUM; MEMBRANES; DISEASE; GROWTH;

D O I：

10.3390/mi11020150

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.

引用

页数：16

共 58 条

[1] Growth of intestinal epithelium in organ culture is dependent on EGF signalling [J].