Illustrating the concepts of isotopes and mass Spectrometry in introductory courses: A MALDI-TOF mass Spectrometry laboratory experiment

Students completing this lab gain hands-on experience using a mass spectrometer and learn about the requirements for mass spectral analysis and the consequences of isotopes. The data analysis reinforces concepts often taught early in general chemistry courses. We continue to expose students to mass spectrometry throughout their undergraduate careers with the goal of providing them the opportunity to assimilate the chemistry, usefulness and beauty of MALDI mass spectrometry. The lab could be adapted to any of the soft ionization techniques, depending upon what is available or preferred. Changes to the sample preparation and data collection procedure could easily be made to accommodate a different technique.