Protective effect of liquiritin on corticosterone-induced neurotoxicity in PC12 cells

被引：4
作者：
Wu, Jing [1 ]
Huang, Fei Xiang [2 ]
Wang, Jing [3 ]
Shi, Chang Cheng [4 ]
Fang, Guo Ying [1 ]
机构：
[1] Hangzhou Obstet & Gynecol Hosp, Dept Pharm, Hangzhou 310008, Zhejiang, Peoples R China
[2] Hangzhou Obstet & Gynecol Hosp, Dept Tradit Chinese Med, Hangzhou 310008, Zhejiang, Peoples R China
[3] Hangzhou Obstet & Gynecol Hosp, Dept Obstet, Hangzhou 310008, Zhejiang, Peoples R China
[4] Hangzhou First Peoples Hosp, Dept Pharm, Hangzhou, Zhejiang, Peoples R China
来源：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2018年 / 17卷 / 10期
关键词：
Liquiritin; Antidepressant; Corticosterone; Neuroprotection; PC12; cells; BDNF/TrkB signaling; NEUROTROPHIC FACTOR BDNF; SIGNALING PATHWAYS; DEPRESSED-PATIENTS; MICE; APOPTOSIS; RATS;
D O I：
10.4314/tjpr.v17i10.17
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Purpose: To determine the protective effects of liquiritin on corticosterone-induced neurotoxicity in rat pheochromocytoma (PC12) cells. Methods: Neurotoxicity in PC12 cells was induced by different concentrations of corticosterone. Proliferation of PC12 cells was evaluated using CCK8 assay kits, while apoptosis was determined by flow cytometry. Results: The results indicate that corticosterone inhibited the proliferation of PC12 cells time- and dose-dependently. The inhibitory effect (0.2 mM) was ameliorated by liquiritin. Furthermore, the cell apoptosis rate and protein level of caspase 3 in PC12 cells induced by corticosterone were ameliorated by liquiritin (1 and 2 mg/mL) treatment. Moreover, the protective effect of liquiritin (2 mg/mL) on corticosterone induced neurotoxicity in PC12 cells was weakened by K252a (the specific TrkB inhibitor) treatment. In addition, the protein level of brain-derived neurotrophic factor (BDNF) and (tyrosine-kinase receptor) TrkB showed a reverse trend to caspase 3. Conclusion: Liquiritin shows protective effects against neurotoxicity induced by corticosterone in PC12 cells, and these effects are exerted via up-regulating BDNF/TrkB signaling.
引用
收藏
页码：2013 / 2017
页数：5
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