Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

被引：47

作者：

Cho, Heewon ^{[1
]}

Kwon, Haw-Young ^{[2
]}

Sharma, Amit ^{[3
]}

Lee, Sun Hyeok ^{[1
]}

Liu, Xiao ^{[4
]}

Miyamoto, Naoki ^{[2
]}

Kim, Jong-Jin ^{[5
]}

Im, Sin-Hyeog ^{[3
,6
,7
]}

Kang, Nam-Young ^{[8
]}

Chang, Young-Tae ^{[1
,2
,4
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 37673, South Korea

[2] Inst Basic Sci IBS, Ctr Self Assembly & Complex, Pohang 37673, South Korea

[3] Pohang Univ Sci & Technol POSTECH, Dept Life Sci, Pohang 37673, South Korea

[4] Pohang Univ Sci & Technol POSTECH, Dept Chem, Pohang 37673, South Korea

[5] Sunchon Natl Univ, Dept Biol, Sunchon 57922, South Korea

[6] ImmunoBiome Inc, Pohang 37666, South Korea

[7] Yonsei Univ, Inst Convergence Res & Educ Adv Technol, Seoul 03722, South Korea

[8] Pohang Univ Sci & Technol POSTECH, Dept Convergence IT Engn, Pohang 37673, South Korea

来源：

NATURE COMMUNICATIONS | 2022年 / 13卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

POLARIZATION;

D O I：

10.1038/s41467-022-33526-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Studying the specific roles of macrophage subsets has been hampered by a lack of subset-specific probes. Here the authors report an M1 selective fluorescent probe named CDr17, and demonstrate the suitability of this probe for tracking M1 macrophages in vivo. Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naive M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

引用

页数：8

共 33 条

[1] Development of a Highly Sensitive Fluorescence Probe for Hydrogen Peroxide [J].