Development of a whole cell biosensor for the detection of inorganic mercury

被引：26
作者：
Mahbub, Khandaker Rayhan
Krishnan, Kannan
Naidu, Ravi
Megharaj, Mallavarapu
机构：
[1] Univ Newcastle, Fac Sci & Informat Technol, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia
[2] CRC CARE, Callaghan, NSW 2308, Australia
来源：
ENVIRONMENTAL TECHNOLOGY & INNOVATION | 2017年 / 8卷
关键词：
bioavailable metal; gfp; detection; toxicity; Sphingobium; BACTERIAL SENSORS; ENVIRONMENT; SEDIMENTS; SAMPLES; HG(II); SOILS; GFP;
D O I：
10.1016/j.eti.2017.04.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A whole cell bacterial biosensor was developed by modification of chromosomal DNA of an environmental bacterial isolate Sphingobium SA2. The sensing element contained green fluorescence protein gene gfp fused to short segment of merA gene of Sphingobium SA2. The sensing element was introduced into electro-competent cells of Sphingobium SA2, where it integrated into the bacterial DNA due to homologous recombination. The transformed cells were able to produce green fluorescence in 5 h in the presence of nano-molar concentrations of mercury. A linear positive correlation was observed between 0-40 nm Hg and fluorescence intensity. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：64 / 70
页数：7
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