Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

被引:14
作者
Ordonez-Garcia, Magali [1 ]
Rios-Velasco, Claudio [1 ]
de Jesus Ornelas-Paz, Jose [1 ]
Carlos Bustillos-Rodriguez, Juan [1 ]
Horacio Acosta-Muniz, Carlos [1 ]
Ignacio Berlanga-Reyes, David [1 ]
Angel Salas-Marina, Miguel [2 ]
Jhonathan Cambero-Campos, Octavio [3 ]
Gallegos-Morales, Gabriel [4 ]
机构
[1] Ctr Invest Alimentac & Desarrollo AC, Unidad Cuauhtemoc, Ave Rio Conchos S-N Parque Ind, Cuauhtemoc 31570, Chihuahua, Mexico
[2] Univ Ciencias & Artes Chiapas, Unidad Acad Villacorzo, Fac Ingn, Chiapas, Mexico
[3] Univ Autonoma Nayarit, Unidad Acad Agr, Nayarit, Mexico
[4] Univ Autonoma Agr Antonio Narro, Dept Parasitol Agr, Saltillo, Coahuila, Mexico
关键词
baculovirus; biological control; fall armyworm; genetic variability; morphology; virulence; HELICOVERPA-ARMIGERA; LITURA LEPIDOPTERA; FALL ARMYWORM; BACULOVIRUSES; RESISTANCE; EFFICACY; ABILITY; PESTS;
D O I
10.1111/jen.12715
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), is the most important pest of maize in many countries. Entomopathogenic viruses mainly Baculoviruses family are excellent biological control agents and therefore a viable alternative for managing this pest. The aim of this study was to determine the biological activity of eight native nucleopolyhedrovirus (NPVs) against FAW larvae. Additionally, two of the most virulent isolates (SfCH32 and SfCH15) were characterized biologically by bioassays to estimate their median lethal dose (LD50) and median lethal time (LT50), morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) and molecularly by restriction enzymes. Three (SfCH15, SfCH18 and SfCH32) of the eight tested native nucleopolyhedrovirus isolates caused mortalities >98% at 168-hr post-inoculation (hpi) with a dose of 9.2 x 10(4) OBs/larva. SfCH15 and SfCH32 isolates showed occlusion bodies (OBs) of irregular shape and size (1.02-2.24 mu m). The SfCH15 and SfCH32 isolates showed similar median lethal dose (5.6 x 10(2)-6.4 x 10(2) OBs/larva). The lowest median lethal time (114.5 hpi) was observed with the SfCH15 isolate at the highest concentration (2.5 x 10(6) OBs/larva). The DNA restriction profiles for SfCH15 and SfCH32 were different, with their genome size being 128,000 bp and 132,000 bp, respectively. SfCH15 and SfCH32 isolates showed similar morphological characteristics and the highest virulence against fall armyworm. This study showed that native isolates were highly virulent against S. frugiperda larvae, being similar to other reported strains; however, field studies are required to confirm their insecticidal effect.
引用
收藏
页码:123 / 132
页数:10
相关论文
共 45 条
[1]   A method of computing the effectiveness of an insecticide [J].
Abbott, WS .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1925, 18 :265-267
[2]   Lethal and sub-lethal effects of select macrocyclic lactones insecticides on forager worker honey bees under laboratory experimental conditions [J].
Abdu-Allah, Gamal A. M. ;
Pittendrigh, Barry R. .
ECOTOXICOLOGY, 2018, 27 (01) :81-88
[3]   Resistance to new insecticides and their synergism in Spodoptera exigua (Lepidoptera: Noctuidae) from Pakistan [J].
Ahmad, Mushtaq ;
Farid, Abid ;
Saeed, Muhammad .
CROP PROTECTION, 2018, 107 :79-86
[4]   Genotype assembly, biological activity and adaptation of spatially separated isolates of Spodoptera litura nucleopolyhedrovirus [J].
Ali, Ghulam ;
Abma-Henkens, Marleen H. C. ;
van der Werf, Wopke ;
Hemerik, Lia ;
Vlak, Just M. .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2018, 153 :20-29
[5]   Spodoptera frugiperda multiple nucleopolyhedrovirus as a potential biological insecticide: Genetic and phenotypic comparison of field isolates from Colombia [J].
Barrera, Gloria ;
Simon, Oihane ;
Villamizar, Laura ;
Williams, Trevor ;
Caballero, Primitivo .
BIOLOGICAL CONTROL, 2011, 58 (02) :113-120
[6]   Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E.!Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD [J].
Barreto, MR ;
Guimaraes, CT ;
Teixeira, FF ;
Paiva, E ;
Valicente, FH .
NEOTROPICAL ENTOMOLOGY, 2005, 34 (01) :67-75
[7]   Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda [J].
Behle, Robert W. ;
Popham, Holly J. R. .
JOURNAL OF INVERTEBRATE PATHOLOGY, 2012, 109 (02) :194-200
[8]   Baculovirus Entry and Egress from Insect Cells [J].
Blissard, Gary W. ;
Theilmann, David A. .
ANNUAL REVIEW OF VIROLOGY, VOL 5, 2018, 5 :113-139
[9]  
Caballero P., 2001, BACULOVIRUS SUS APLI, P15
[10]  
Casmuz Augusto, 2010, Rev. Soc. Entomol. Argent., V69, P209